1. Play with a friend or alone! Paulos Menos II
Lab Midterm Jeopardy
Play with a friend or alone!
Paulos Menos II

2. Some quick things This doesn’t cover everything.
The difficulty distribution of the questions is most likely not equal, I’m not perfect…
Don’t get to behind in studying for test 3 as well, it may come sooner than you realize

3. simply answer the question. happy studying (if possible)!
Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9 10 20 30 40 50

4. Lab 1 10 pts
What does PPE stand for?

5. 10 pts
Personal protective equipment.

6. Lab 1 20 pts
How long should the loop be leaved inside the incenerator?

7. 20 pts
Until ¾ of the loop glows red hot

8. Lab 1 30 pts Which of the following is required PPE for MCB3020 lab?
Lab coat
Goggles
Gloves
Long sleeved shirts

9. 30 pts
Long sleeved shirts

10. Lab 1 40 pts
What BSL is the microbiology lab at UCF?

11. 40 pts
BSL-2

12. Lab 1 50 pts
First aid kits are located where?

13. 50 pts
In the hallway between the labs.

14. Lab 2 10 pts
True or False. When holding a microscope, you only need to hold it at the arm.

15. 10 pts
False. You need to hold the arm and the base.

16. Lab 2 20 pts
____________ is a specialized staining method that uses an anionic dye to stain background rather than the cell.

17. 20 pts
Negative staining

18. Lab 2 30 pts
Nigrosin is an example of what sort of dye?

19. 30 pts
Anionic dyes

20. Lab 2 40 pts
Bacteria that exhibit a variety of cell shapes in a sample are known as ____________?

21. 40 pts
Pleomorphic

22. Lab 2 50 pts
Tetracocci are to tetrads as Sarcinae are to what?

23. 50 pts
Cuboidal packets

24. Lab 3 10 pts
The primary dye in the gram stain is?

25. 10 pts
Crystal Violet

26. Lab 3 20 points
What is the mordant used in the Gram stain?

27. 20 points
Gram’s Iodine

28. Lab 3 30 pts
Paulos is performing a Gram stain. He uses the primary dye but gets distracted and forgets to complete the rest of the steps. What color would the bacteria appear to be under his microscope?

29. 30 pts
Purple

30. Lab 3 40 pts
The Gram stain is a __________ stain.

31. 40 pts
Differential

32. Lab 3 50 pts What is the decolorizer in the Gram stain? dH2O
70% Ethanol
H2O
95% Ethanol

33. 50 pts
95% Ethanol

34. Lab 4 10 PTS
Methylene blue will dye cells what color?

35. 10 pts
Blue

36. Lab 4 20 pts
True or False. Steaming is required after the secondary dye is used to stain the cell in Acid-Fast stain.

37. 20 pts
False.

38. Lab 4 30 pts
What is the decolorizer in the endospore stain?

39. 30 pts
Water

40. Lab 4 40 pts
Ziehl-Neelsen uses what dye as it’s primary dye?

41. 40 pts
Carbol fuchsin.

42. Lab 4 50 pts
Which of the following is matched correctly for the Schaeffer-Fulton Method?
Malachite green and Methylene blue
Carbol fuchsin and Safranin
Malachite green and Safranin
Carbol fuchsin and Methylene blue
Crystal violet and Safranin

43. 50 pts
Malachite green and Safranin

44. Lab 5 10 pts
The solid, gelatin-like substance used in petri dishes is known as what?

45. 10 pts
Agar

46. Lab 5 20 pts
True or False. You need to sterilize your loop when streaking from different zones on the same petri dish.

47. 20 pts
True.

48. Lab 5 30 pts
In which of the zones on a streak plate should you find isolation?
Zone I
Zone II
Zone III
Zone IIIB

49. 30 pts
Zone IIIB.

50. Lab 5 40 pts
What would the dilution factor be?
1 ml
7 ml
9 ml

51. 40 pts
1/10

52. Lab 5 50 pts C
If you needed to isolate bacteria that formed small white colonies on this agar, which CFU should you choose? A B D

53. 50 pts D D

54. Lab 6 10 pts
_______ is characterized with complete clearing of the red blood cells.

55. 10 pts
Beta (β) hemolysis

56. Lab 6 20 pts
Cloudiness around the stab line in motility is a (positive, negative) result.

57. 20 pts
Positive

58. Lab 6 30 pts
What inhibits the growth of Gram negative organisms in PEA?

59. 30 pts
Phenylethyl Alcohol

60. Lab 6 40
___________ fermenters produce light purple to pink colonies on EMB agar.

61. 40 pts
2,3-butanediol

62. Lab 6 50 pts
What percentage of agar is added into motility medium?

63. 50 pts
0.4%

64. Lab 7 10 pts
What does TNTC mean?

65. 10 pts
Too numerous to count

66. Lab 7 20 pts
What does TFTC mean?

67. 20 pts
Too few to count

68. Lab 7 30 pts
What does CFU stand for?

69. 30 pts
Colony forming units.

70. Lab 7 40 pts
What is the viable counting range for CFU’s on a plate.

71. 40 pts
30–300

72. Lab 7 50 pts
What is the dilution in the 6 mL tube?

73. 50 pts
20 CFU/mL
This question is set up to be a bit more difficult than anything you would see on a lab exam so you guys can get a bit more practice

74. Lab 8 10 pts
A clearing zone around a colony on milk agar is a (positive, negative) result.

75. 10 pts
Positive

76. Lab 8 20 pts
In the Phenol Red Broth, ________ tubes can be used to see if gas production occurred.

77. 20 pts
Durham

78. Lab 8 30 pts
True or False. Lactose is read at the bottom of the tube in KIA.

79. 30 pts
False

80. Lab 8 40
KIA is ideally read about how long after its been inoculated.

81. 40 pts
18 hours

82. Lab 8 50
What percentage of glucose is found in KIA?

83. 50 pts
0.1%

84. Lab 9 10
True or Fasle? A positive result in the gelatin test will have the medium liquefied after sitting 20 minutes in ice.

85. 10 pts
True

86. Lab 9 20
When the Tryptone broth is used, a positive result will turn the top of the tube what color?

87. 20 pts
Cerise

88. Lab 9 30
True or false? You stab the agar in a Phenylalanine Slant.

89. 30 pts
False

90. Lab 9 40
In the Simmon’s Citrate test, a positive result turns the media what color?

91. 40 pts
Blue

92. Lab 9 50
With the Methyl red tests in the MR-VP, a _____________ fermenter will turn the broth to a red color.

93. 50 pts
Mixed acid fermenter