1. Nuclear export of endogenous APC in different cell lines.
Nuclear export of endogenous APC in different cell lines. (A) SW480 cells were treated ±5 h LMB, fixed with formalin and stained with antibodies Ali 12–28 (1:50) and Ab7 (1:50) followed by Texas red secondary conjugate. LMB induced a visible shift to the nucleus as shown by IF. The nucleus is stained with Hoechst chromatin dye. (B) Nuclear and cytoplasmic cell extracts were prepared from SW480 (APCmut/mut), HT29 (APCmut/mut) and HCT116 (APCwt/wt) colon cancer cell lines. The endogenous truncated or full‐length forms of APC were separated by SDS–PAGE (or agarose gels for HCT116 extracts, see Methods) and detected by western blot using M‐APC antibody. The APC bands were quantified by densitometry and the cytoplasmic/nuclear ratios and LMB‐dependent nuclear shift are shown. Similar results were observed in two independent experiments.
Mariana Brocardo et al. EMBO Rep. 2005;6:
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