1. Effects of sodium chloride on algal growth
PJAS
Effects of sodium chloride on algal growth
Nate Kazienko
Pittsburgh Central Catholic
Grade 9
When another Central student is presenting:
Write a critique on their PowerPoint
WRITE DOWN WHAT THE JUDGES ASK AND THE PERSONS EXACT RESPONSE

2. Surface Runoff Part of the water cycle Impacts aquatic life
Commonly pollutes lakes, streams, and rivers
Pollutants include: fertilizers, salt, oil, and sediment

3. Introduction Variable: Sodium Chloride Euglena and Chlamydomonas
Inorganic compound
Commonly referred to as salt
Major surface runoff pollutant
Over 20 million tons of salt is used on roads in the US every year
Euglena and Chlamydomonas
Two common species of algae

4. Effects of Sodium Chloride on Aquatic Life
Enters bodies of water through runoff
Causes an eutrophication effect
Prevents dissolved oxygen from reaching the bottom layers
Causes oxygen depletion in bottom layers
Results in fish mortality
Mention how SC messes with tonicity of algae
Also mention how an OD of salt can kill everything

5. Algal Models Euglena gracilis Chlamydomonas reinhardtii One flagellum
Found in freshwater and saltwater
40-60um long
Multiple chloroplasts
Chlamydomonas reinhardtii
Two flagella
Found in freshwater and saltwater
Normally around 10um wide
Large chloroplast

6. Purpose
To determine the effects of various sodium chloride concentrations on the growth of Euglena and Chlamydomonas

7. Hypotheses
Null: Sodium chloride will not have a significant effect on the growth of Euglena and Chlamydomonas
Alternative: Sodium chloride will have a significant effect on the growth of Euglena and Chlamydomonas
Only state alternative
Null was used for stats

8. Materials Culture tube rack 25 culture tubes (13x100 mm borosilicate)
200μl micro-pipette
1000μl micro-pipette
Carolina Spectrophotometer
Euglena gracilis (from Carolina)
Chlamydomonas reinhardtii (from Carolina)
Spring water
Soil water (from Carolina)
Sodium chloride
Pipette tips
Only mention highlighted materials
Wait 5 seconds after speaking before next slide

9. Procedure
Arranged 24 test tubes in the rack to receive equal amounts of sunlight
Pipetted 2ml of Chlamydomonas into 12 test tubes and 1ml of Euglena into the remaining 12 test tubes (Euglena is more populous)
Created sodium chloride concentrations according to the chart on the next slide
Recorded absorbance on days 0,2,4,6,8,10,12, and 14 using the spectrophotometer set at a 430nm wavelength
Say they were recorded every other day for 2 weeks
Say you mixed the concentrations

10. Procedure (Continued)
Concentration 0%
0.1% 1%
Chlamydomonas
2mL
Soil Water
1mL
Spring Water
1.95mL
1.5mL
Sodium Chloride
0mL
0.05mL
0.5mL
Total
5mL

11. Procedure (Continued)
Concentration 0%
0.1% 1%
Euglena
1mL
Soil Water
Spring Water
3mL
2.95mL
2.5mL
Sodium Chloride
0mL
0.05mL
0.5mL
Total
5mL
Don't mention this chart

12. Day 14 P-Value: 0.999 Day 6 P-Value: 0.866
Data is all the judges care about

13. Day 14 P-Value: 0.114
Day 6 P-Value: 0.016

14. Results
The null hypothesis that the Euglena would not vary from the control cannot be rejected (P-Value>0.05) for all day 6 and day 14 concentrations. The null hypothesis that the Chlamydomonas would not vary from the control cannot be rejected (P-Value>0.05) for all day 6 and day 14 concentrations.

15. Conclusion
The null hypothesis is accepted because neither the Euglena or Chlamydomonas significantly varied from the control.

16. Limitations Only two models of algae used Short experiment time
More readings
Health of the species?
No cell counts preformed
Optimal conditions for experiment?

17. Extensions Preform a viable cell count Longer test duration
Preform experiment in optimal environment

18. Sources
reduction-initiative/impacts.htm
measurements/parameters/water-quality/dissolved-oxygen/#1

19. Euglena ANOVA Day 6

20. Euglena ANOVA Day 14

21. Chlamydomonas ANOVA Day 6

22. Chlamydomonas ANOVA Day 14