1. Atp1a3 surface biotinylation.
Atp1a3 surface biotinylation. (A) YFP-β1 (black) or YFP-β2 (red) were co-transfected with HA-tagged Atp1a3 WT, TA, and TD in HEK 293T cells. Surface proteins were labelled with biotin, washed, and isolated with streptavidin beads. Graph data represent means ± SEM, n = 6 independent experiments with β1, n = 4 independent experiments with β2. β1/TA P = 5.8 × 10−8, β1/TD P = 2.8 × 10−14, β2/TA P = 4 × 10−6, β2/TD P = 2.8 × 10−5; ****P < , t test.
Amy W Lin et al. LSA 2018;1:e
© 2018 Lin et al.