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Intestinal phosphate absorption in a model of chronic renal failure

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1 Intestinal phosphate absorption in a model of chronic renal failure
J. Marks, L.J. Churchill, S.K. Srai, J. Biber, H. Murer, P. Jaeger, E.S. Debnam, R.J. Unwin  Kidney International  Volume 72, Issue 2, Pages (July 2007) DOI: /sj.ki Copyright © 2007 International Society of Nephrology Terms and Conditions

2 Figure 1 Autoradiography (left section) and light field (right section) images of duodenum and jejunum from sham and 5/6-nephrectomy animals (bar=100 μm). Uptake was performed in vitro for 2 min using 100 μM32P phosphate. Kidney International  , DOI: ( /sj.ki ) Copyright © 2007 International Society of Nephrology Terms and Conditions

3 Figure 2 In vivo uptake of phosphate by the duodenum and jejunum of sham-operated and 5/6-nephrectomized animals. Results are expressed as mean±s.e.m. of six experiments per region as a percentage of the initial counts transferred from 5 cm of small intestine into 1 ml of blood. **P<0.01, compared with sham jejunum and #P<0.05 compared with 5/6-nephrectomy jejunum using a one-way analysis of variance with post hoc comparisons performed using the Bonferroni multiple comparisons test. Kidney International  , DOI: ( /sj.ki ) Copyright © 2007 International Society of Nephrology Terms and Conditions

4 Figure 3 Mucosal retention of phosphate in the duodenum and jejunum of sham-operated and 5/6-nephrectomized animals. Results are expressed as mean±s.e.m. of six experiments per region in nmol/g mucosa. *P<0.05, **P<0.01 compared with duodenum using a one-way analysis of variance with post hoc comparisons performed using the Bonferroni multiple comparisons test. Kidney International  , DOI: ( /sj.ki ) Copyright © 2007 International Society of Nephrology Terms and Conditions

5 Figure 4 Real-time PCR quantification of NaPi-IIa mRNA in the kidney of sham and 5/6-nephrectomized rats. Results are expressed as the mean±s.e.m. of duplicate PCR reactions performed on six individual samples. The abundance of NaPi-IIa is given as a ratio of NaPi-IIa mRNA to β-actin mRNA in arbitrary units (AU). ***P<0.001 using an unpaired t-test. Kidney International  , DOI: ( /sj.ki ) Copyright © 2007 International Society of Nephrology Terms and Conditions

6 Figure 5 Real-time PCR quantification of NaPi-IIb mRNA in the duodenum and jejunum of sham and 5/6-nephrectomized rats. Results are expressed as the mean±s.e.m. of duplicate PCR reactions performed on six individual samples. The abundance of NaPi-IIb is given as a ratio of NaPi-IIb mRNA to β-actin mRNA in arbitrary units (AU). Kidney International  , DOI: ( /sj.ki ) Copyright © 2007 International Society of Nephrology Terms and Conditions

7 Figure 6 Real-time PCR quantification of NaPi-IIa mRNA in the kidney of 5/6-nephrectomized rats maintained on a normal or low-phosphate diet for 10 days. Results are expressed as the mean±s.e.m. of duplicate PCR reactions performed on six individual samples. The abundance of NaPi-IIa is given as a ratio of NaPi-IIa mRNA to β-actin mRNA, in arbitrary units (AU). **P<0.01 using an unpaired t-test. Kidney International  , DOI: ( /sj.ki ) Copyright © 2007 International Society of Nephrology Terms and Conditions

8 Figure 7 In vivo uptake of phosphate by the jejunum of 5/6-nephrectomized animals maintained on a normal or a low-phosphate diet for 10 days. Results are expressed as mean±s.e.m. of six experiments as a percentage of the initial counts transferred from 5 cm of small intestine into 1 ml of blood. Kidney International  , DOI: ( /sj.ki ) Copyright © 2007 International Society of Nephrology Terms and Conditions

9 Figure 8 Real-time PCR quantification of NaPi-IIb mRNA in the duodenum and jejunum of 5/6-nephrectomized rats maintained on a normal or low-phosphate diet for 10 days. Results are expressed as the mean±s.e.m. of duplicate PCR reactions performed on six individual samples. The abundance of NaPi-IIb is given as a ratio of NaPi-IIb mRNA to β-actin mRNA in arbitrary units (AU). Kidney International  , DOI: ( /sj.ki ) Copyright © 2007 International Society of Nephrology Terms and Conditions


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