1. Barley (Hordeum vulgare subsp. vulgare)
2n = 2x = 14
~5,000 Mb
Long history of genetics research
Economically important crop
Malting/brewing, food, feed, forage
Model for polyploid Triticeae

2. Oregon Wolfe Barley Mapping Population
Doubled haploid
International open resource
Morphological, isozyme, RFLP, SSR, AFLP, DArT, SNP data
Linkage maps
QTLs

3. Considerations in mapping:
a RAD case study

4. Why the OWB? Maximum polymorphism and anchor markers

5. RAD maps compared to prior maps
# of linkage groups ✔
Order ✔
Distance ✔
Gap filling ~

6. RAD maps compared to prior maps
Quality ✔

7. RAD maps and compatibility
Integration with prior data ✔

8. RAD maps and applications
Utility ✔

9. RAD maps and applications
Synteny ✔

10. Uses of linkage maps and QTLs
Marker Assisted Selection
Genome Wide Association Scans
Genomic Selection
Isolation and/manipulation of candidate genes

11. Ideal markers for molecular plant breeding
Codominant
Consistent genome coordinates across accessions
Discriminate between gene family members
Gene-based (and/or) based on functional determinants of phenotype
Integrated with prior data

12. Ideal genotyping service for plant breeding
DNA extraction included – seed chip ideal
Quick turnaround
Cost

13. Linkage maps – souvenir horseshoes or workhorses?
Breeders make crosses
Actual recombination is critical
Detect chromosome rearrangements

14. Acknowledgements: Generations of OWB practitioners
OWB-dominant x OWB recessive F1
Doubled haploids