1. THE NQAD IS HIGHLY SENSITIVE, ALLOWING MORE SAMPLE COMPONENTS TO BE DETECTED
Application note # QAN-0058
CHROMATOGRAMS
FLOW RATE: 1.0 mL/min
COLUMN: Thermo BetaBasic C18, 150 x 4.6 mm, 5u
COLUMN TEMP: Ambient
INJECTION VOLUME :10 µL
ANALYTE: Hydrocortisone
NQAD
GAS: Air
FILTER: 1.3 sec
GAIN: 1X
EVAPORATION TEMP: 35C
ELSD
GAS: Nitrogen, 1.5 L/min
GAIN: 1
EVAPORATION TEMP: 40C
HPLC
MOBILE PHASE: 45:55 water:ACN
PARAMETERS
0.00
0.20
0.40
0.60
0.80
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
2.60
2.80
3.00
4.00
9.00 mV
min 1
NQAD, 10 ng
Avg S/N, 53.5
APPLICATION NOTES 2
0.00
0.20
0.40
0.60
0.80
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
2.60
2.80
3.00
45.00
48.00 mV
min 1
ELSD, 10 ng
Avg S/N, 2.8
Demonstrates better sensitivity than ELSD ™
QUANT TECHNOLOGIES, LLC
TH Lane NE
Blaine, MN, USA 2
1 = Injection Artifact, 2 = Hydrocortisone, 10 ng 1