1. The SOP generates high-quality full-genome sequence data across multiple ssRNA+ virus families.
The SOP generates high-quality full-genome sequence data across multiple ssRNA+ virus families. Pooled samples from the SOP were sequenced on the Illumina MiSeq platform. Samples were demultiplexed, the adaptors were trimmed, and low-quality sequencing reads were removed. Sequencing reads were mapped corresponding to input viruses. These viruses include foot-and-mouth disease virus (FMDV) type O (GenBank accession no. KF ) (A), West Nile virus (WNV) AF ) (B), human rhinovirus 16 (HRV-16) (GenBank accession no. L ) (C), Chikungunya virus (CHIKV) (pJM6-3-CHIKV 181/25-mkate) (D), and Middle East respiratory syndrome coronavirus (MERS) (GenBank accession no. KJ ) (E). Nucleotide coverage depth (NT coverage) is indicated on the y axis, and nucleotide (NT) position is indicated on the x axis. The genome length for each virus is indicated on the x axis, and the percentage of the genome covered greater than 3 nucleotides is indicated. For FMDV, WNV, HRV-16, and CHIKV, data represent material from a single barcode. For MERS, the data shown is a combination of four barcodes generated from the same sample.
Lindsey A. Moser et al. mSystems 2016; doi: /mSystems