1. Expression, Candidate Gene, and Population Studies of the Melanocortin 5 Receptor 
Naohito Hatta, Craig Dixon, Amanda J. Ray, Sion R. Phillips, William J. Cunliffe, Mike Dale, Carol Todd, Simon Meggit, Mark A. Birch- Machin, Jonathan L. Rees 
Journal of Investigative Dermatology 
Volume 116, Issue 4, Pages (April 2001)
DOI: /j x x
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Specificity of the anti-MC5-R antibody (C-18). Immunocytochemistry of (a) cells transfected with the MC5-R gene, (b) untransfected cells, and (c) cells transfected with the MC1-R gene. Scale bars, 100 µm.
Journal of Investigative Dermatology  , DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Expression of the MC5-R protein in normal skin. Immunohistochemistry of MC5-R in normal skin using a polyclonal antibody (C-18). (a) Epidermal keratinocytes show low level expression (at the limits of detection when photographed) of MC5-R, and epithelium of (b) eccrine glands, (c) apocrine glands, and (d) arrector pili muscle show strong expression of MC5-R. Scale bars, 100 µm.
Journal of Investigative Dermatology  , DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Expression of MC5-R mRNA detected by RT-PCR in normal human skin and cultured cells. Upper panel: 227 bp RT-PCR products using primers specific for MC5-R. Lower panel: 254 bp RT-PCR products using primers specific for the β-actin gene as controls. M = 100 bp DNA marker. Lane 1, normal human skin; lane 2, upper portion of microdissected skin; lane 3, lower portion of microdissected skin; lane 4, cultured normal human keratinocytes; lane 5, immortalized human keratinocytes (HaCaT); lane 6, cultured normal human melanocytes; lane 7, cultured normal human fibroblasts.
Journal of Investigative Dermatology  , DOI: ( /j x x)
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5. Figure 4
Comparison of the amino acid sequences of human, chimpanzee, and murine MC5-R. The human consensus sequence was taken as that which is most common in each of the populations. The chimpanzee sequence was obtained from genomic DNA by PCR with primers specific for human MC5-R. The mouse sequence was obtained from GenBank (Accession number X76295,Labbe et al, 1994). The two nonsynonymous changes between the human and chimpanzee sequences are marked (amino acid numbers 224 and 272) and the location of the only nonsynonymous polymorphism (Phe209Leu) in the human population is shown. The locations of the seven transmembrane domains are shown (TM1−TM7).
Journal of Investigative Dermatology  , DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
Gene trees of the MC5-R gene in African, Asian, and Caucasian populations. (a) African, (b) Asian, and (c) Caucasian populations. Closed circles represent the MC5-R consensus sequence, open circles represent synonymous changes, and dotted circles represent nonsynonymous changes. The size of each circle is proportional to the relative allele frequency.
Journal of Investigative Dermatology  , DOI: ( /j x x)
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7. Figure 6
Intracellular cAMP levels in HEK-293 cells transfected with either wild-type or Phe209Leu variant MC5-R, in response to α-MSH. Intracellular cAMP levels were measured per 105 cells. The results of cells transfected with wild-type and Phe209Leu variant MC5-R are shown by closed and open squares, respectively. Each experiment was performed four times and this graph displays data from a single representative experiment.
Journal of Investigative Dermatology  , DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions