1. (Life Sciences College, Nankai University )
The cloning of the Brine Shrimp DNA fragments correlated to the Bombyx mori doublesex gene
Zeng Hui
(Life Sciences College, Nankai University )

2. >1.0
0.5

3. The sex-determination system differs extremely among organisms.
sex chromosomes , XX/XY of ZZ/ZW
autosomal genes , (diploid/haploid sex-determination system )
environmental and other epigenetic factors
However, the study of the dsx gene has shown an apparent exception.

4. The homologues of dsx have been isolated in the silkworm, mouse, human, chicken and other insects. The mab-3 gene of Caenorhabditis elegans is also the structural homologue of dsx.
Mouse,chicken
Human
Nematode
Silkworm
mab-3
Bmdsx
Dmrt1
DMRT1
homologues of dsx

6. The following amplification showed that this pair of primers could make two visible bands appear from genomic DNA of Artemia parthenogenetica from Gahai Lake, China. The two bands were named Apdsx900 and Apdsx200 respectively.
1. DNA 1000bp~100bp ladder, 2. genomic DNA of Artemia sinica ,
3. genomic DNA of Artemia parthenogenetica, 4. λDNA/Hind III+EcoR I
Apdsx900
Apdsx200

7. 1. Artemia parthenogenetica, 2. Artemia sinica
Apdsx200 were labeled as probe with DIG and hybridized respectively to the genomic DNA of Artemia parthenogenetica and Artemia sinica digested by restriction endonucease HaeIII.
1. Artemia parthenogenetica, 2. Artemia sinica
The signals indicated that the Apdsx200 was a low copy DNA sequence.

8. The sequences of Apdsx900

9. Further analysis was required to assure the expression of the sequence of Apdsx900 in Artemia parthenogenetica bodies and localize it onto chromosomes of Artemia parthenogenetica.

10. Thank you very much!