1. Zinc protects sperm from being damaged by reactive oxygen species in assisted reproduction techniques 
Jinxiang Wu, Shiqiang Wu, Yuanzhi Xie, Zhengyao Wang, Ruiyun Wu, Junfeng Cai, Xiangmin Luo, Suzhen Huang, Liuxia You 
Reproductive BioMedicine Online 
Volume 30, Issue 4, Pages (April 2015)
DOI: /j.rbmo
Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

2. Figure 1
Evaluation of the extent of sperm DNA damage. Sperm DNA fragmentation (SDF): with no halo or thickness of unilateral halo less than one-third of the minimum diameter of the sperm head. Sperm DNA integrity (SDI): large halo or thickness of unilateral halo is larger than one-third of the minimum diameter of the sperm head indicating intact DNA.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

3. Figure 2
Sperm membrane integrity in each experimental group (n = 20) after 5 h and 24 h of incubation. Values are presented as mean ± SD. Experimental groups include spermatozoa without ZnCl2 and H2O2 (control); spermatozoa with 0.001% H2O2 (H2O2); spermatozoa with 12.5 nmol/l ZnCl2 (ZnCl2); and spermatozoa incubated successively with 12.5 nmol/l ZnCl2 and 0.001% H2O2 (ZnCl2 + H2O2). aP < 0.05, compared with control; bP < 0.05, comparison with group H2O2.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

4. Figure 3
Evaluation of sperm DNA damage in different experimental groups (n = 20) after 5 h and 24 h of incubation. Values are presented as mean ± SD. Experimental groups include spermatozoa without ZnCl2 and H2O2 (control); spermatozoa with 0.001% H2O2 (H2O2); spermatozoa with 12.5 nmol/l ZnCl2 (ZnCl2); spermatozoa incubated successively with 12.5 nmol/l ZnCl2 and 0.001% H2O2 (ZnCl2 + H2O2).aP < 0.05, compared with control. bP < 0.05, comparison with group H2O2.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions