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Adventitial endothelial implants reduce matrix metalloproteinase-2 expression and increase luminal diameter in porcine arteriovenous grafts  Helen M.

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Presentation on theme: "Adventitial endothelial implants reduce matrix metalloproteinase-2 expression and increase luminal diameter in porcine arteriovenous grafts  Helen M."— Presentation transcript:

1 Adventitial endothelial implants reduce matrix metalloproteinase-2 expression and increase luminal diameter in porcine arteriovenous grafts  Helen M. Nugent, PhD, Robert Tjin Tham Sjin, PhD, Desmond White, BS, Luther G. Milton, LATG, Roberto J. Manson, MD, Jeffrey H. Lawson, MD, PhD, Elazer R. Edelman, MD, PhD  Journal of Vascular Surgery  Volume 46, Issue 3, Pages e2 (September 2007) DOI: /j.jvs Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 A, Schematic diagram of arteriovenous graft and venous implant positioning. The arrows indicate the direction of blood flow. PTFE, Polytetrafluoroethylene. B, Photograph after implantation of three porcine aortic endothelial cell/Gelfoam matrices at the venous site immediately after arteriovenous graft creation. Journal of Vascular Surgery  , e2DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Representative angiograms and measurements. A, Baseline angiograms of control and treated grafts show no significant changes in venous diameter or percentage of stenosis 3 days after surgery. Comparison of angiograms obtained 28 days after surgery compared to baseline show greater venous luminal gain in veins treated with porcine aortic endothelial (PAE) cells and less stenosis at the venous anastomotic site compared with control. B, Bar graph shows changes in venous lumen diameter 3 and 28 days after surgery. C, Bar graph shows the average percentage of stenosis at 3 and 28 days after surgery. Data in B and C are presented with the standard error. *P < .05 compared with day 0; †P < .05 compared with control at the same time point. Journal of Vascular Surgery  , e2DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Representative photomicrographs of hematoxylin and eosin and Verhoeff elastin stained venous cross-sections on days 3 and 28. Increased neovascularization (arrows) was observed in control veins at 3 and 28 days. On day 28, intimal formation occurred predominately at the venous side of the anastomosis. I, Intima; M, media; L, lumen; ADV, adventitia. Journal of Vascular Surgery  , e2DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 Matrix metalloproteinase-2 (MMP-2) localization in porcine jugular veins 3 and 28 days after surgery. A, Media (M) of a control vein with significant MMP-2 expression (arrows) compared with a vein treated with porcine aortic endothelial (PAE) cells 3 days after surgery. Increased MMP-2 expression was also observed at 1 month in control compared with veins treated with PAE cells. I, Intima; ADV, adventitia. B, Quantification of MMP-2 localization. A significant decrease in MMP-2 staining was observed in veins treated with PAE cells compared with control at 3 days. Reduced MMP-2 staining was also observed at 1 month in the intima and media of veins treated with PAE cells compared with control. Data are presented with the standard error. *P < .05 for treatment with PAE cells compared with control. C, Pearson correlation with 95% confidence interval. A strong negative correlation was observed between MMP-2 expression and lumen diameter of veins at 28 days. Journal of Vascular Surgery  , e2DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

6 Fig Matrix metalloproteinase-9 (MMP-9) localization in porcine jugular veins 3 and 28 days after surgery. A, Adventitia (ADV) of a control vein shows increased MMP-9 staining (arrows) 3 days after surgery compared with veins treated with porcine aortic endothelial (PAE) cells at 3 days. Increased MMP-9 expression was also observed in the intima (I) and adventitia at 1 month in veins treated with control Gelfoam compared with veins treated with PAE cells. M, media; L, lumen. B, Quantification of MMP-9 localization presented with the standard error. Reduced MMP-9 expression was observed in the adventitia of veins treated with PAE cells compared with control at 3 days. A decrease in MMP-9 expression was also observed in the intima and adventitia of PAE treated veins compared to control at 1 month. *P < .05 for veins treated with PAE cells compared with control. Journal of Vascular Surgery  , e2DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

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