1. Volume 114, Issue 6, Pages 1287-1295 (June 1998)
Overexpression of pp60c-src elicits invasive behavior in rat colon epithelial cells 
Susan E. Pories, Donald T. Hess, Karen Swenson, Margaret Lotz, Runna Moussa, Glenn Steele, David Shibata, Kimberly M. Rieger- Christ, Ian C. Summerhayes 
Gastroenterology 
Volume 114, Issue 6, Pages (June 1998)
DOI: /S (98)
Copyright © 1998 American Gastroenterological Association Terms and Conditions

2. Fig. 1
In vitro protein kinase activity of pp60c-src performed in the presence of the exogenous substrate casein. Lane 1, FRC/TEX parental cell line; lane 2, FRC/TEXc-src/C; lane 3, FRC/TEXc-src/D; lane 4, FRC/TEXc-src/9; lane 5, FRC/TEXv-src/C. Lane 5 represents a shorter exposure than lanes 1–4 to provide good resolution. The relative kinase activities are represented in histogram format below and assessed after a standard exposure time.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Western blot analysis of avian pp60c-src expression after immunoprecipitation with a panreactive src antibody (Mab 327) probed with an avian-specific src antibody (ECI0). Lane 1, FRC/TEX parental colon epithelial cell line; lane 2, FRC/TEXc-src/9; lane 3, FRC/TEXc-src/B; lane 4, FRC/TEX/hygro/A control; lane 5, FRC/TEXc-src/ C; lane 6, FRC/TEXc-src/D; lane 7, FRC/TEXv-src/C. Black arrowhead shows migrational level of avian c-src; open arrowhead denotes immumoglobulin heavy chain.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Phosphotyrosyl protein profiles in total cell lysates of colon lines. Lane 1, FRC/TEX parental cell line; lane 2, FRC/TEXc-src/9; lane 3, FRC/TEXc-src/C; lane 4, FRC/TEXc-src/D; lane 5, FRC/TEXv-src/C, where one-fifth of the total protein amount was loaded in this lane.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

5. Fig. 4
Morphology of colon epithelial cell lines after introduction and expression of different src constructs. (A) FRC/TEX parental cell line; (B) FRC/TEXc-src/C; (C) FRC/src527/P; (D) FRC/TEXv-src.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

6. Fig. 5
Colony growth in soft agar. (A) FRC/TEX/hygro/A control cell line; (B) FRC/TEXc-src/C; (C) FRC/TEXv-src.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

7. Fig. 6
In vitro (A) migration and (B) invasion assays performed using a panel of rat colon epithelial cells expressing different levels of the c-src proto-oncogene. The parental cell line transfected with the hygromycin gene (FRC/TEX) or the viral src oncogene (FRC/TEXv-src) were used as control cell lines in each assay. FRC/TEX clones 9, C, and D represent three colon epithelial cell transfectants overexpressing c-src.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

8. Fig. 6
In vitro (A) migration and (B) invasion assays performed using a panel of rat colon epithelial cells expressing different levels of the c-src proto-oncogene. The parental cell line transfected with the hygromycin gene (FRC/TEX) or the viral src oncogene (FRC/TEXv-src) were used as control cell lines in each assay. FRC/TEX clones 9, C, and D represent three colon epithelial cell transfectants overexpressing c-src.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

9. Fig. 7
Western blot analysis of total cell lysates probed with antibodies to E-cadherin and α-, β-, and γ-catenin. Lane 1, FRC/TEX control parental rat colon epithelial cell line; lane 2, FRC/TEXc-src/9; lane 3, FRC/TEXc-src/C; lane 4, FRC/TEXc-src/D; lane 5, FRC/TEXv-src.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

10. Fig. 8
Immunoprecipitation of β-catenin from the panel of colon epithelial transfectants (A) probed in Western blot analysis with antiphosphotyrosine antibody and (B) stripped and reprobed with anti–β-catenin antibody. Lane 1, FRC/TEX parental cell line; lane 2, FRC/TEXcsrc/9; lane 3, FRC/TEXc-src/C; lane 4, FRC/TEXc-src/D; lane 5, FRC/TEXv-src; lane 6, FRC/TEX total cell lysate, positive control for phosphotyrosine blot.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions