1. Urethane reduces the frequency of spontaneous recurrent seizures six to eight weeks following 1 h of DFP-induced SE. A, Cortical EEG activity was recorded four to six weeks after SE induction by exposure to DFP. A representative 10-min EEG trace in green fr...
Urethane reduces the frequency of spontaneous recurrent seizures six to eight weeks following 1 h of DFP-induced SE. A, Cortical EEG activity was recorded four to six weeks after SE induction by exposure to DFP. A representative 10-min EEG trace in green from the cortical recording of an adult rat that received diazepam 1 h after SE showed spontaneous rapid increased spike activity that lasted ∼40 s with a fast termination followed by a post-ictal depression. B, A similar EEG trace in green from the cortical recording of a second adult male rat that received urethane 1 h after SE onset showing a similar seizure. Above each raw EEG trace in A, B is a sonogram of the spike activity in the 20- to 70-Hz bandwidth obtained in Spike2. The colors of the sonogram indicate the spectral power density in decibels (dB, inset) at the indicated frequency. C, Diagram showing the total EEG power in the 20- to 70-Hz bandwidth during the 10-min period that includes the SRS in A, B using power analysis software written in Python. The dashed line indicates no spiking activity (baseline). Note that immediately after the spiking stops the baseline of both traces becomes quiet for ∼2 min representing the post-ictal depression. D, E, Heat maps showing the number of seizures detected per day from each rat. F, A difference was detected between the two treatment groups [diazepam (n = 10) and urethane (n = 11)] in the percentage of rats that experienced at least one SRS during the 6–12 d of EEG recording; p = 0.001, Fisher’s exact test. G, A difference was also detected between the average seizure duration for the two treatment groups [diazepam (n = 10) and urethane (n = 11)]. Error bars show SEM; p = 0.01, Student’s t test. The total number of seizures is shown within the bars for each group.
Asheebo Rojas et al. eNeuro 2018;5:ENEURO
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