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Volume 39, Issue 1, Pages (July 2003)

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1 Volume 39, Issue 1, Pages 86-92 (July 2003)
Role of c-kit receptor tyrosine kinase-mediated signal transduction in proliferation of bile epithelial cells in young rats after ligation of bile duct: a study using Ws/Ws c-kit mutant rats  Makoto Satake, Koichi Shimano, Takashi Yamamoto, Atsuhito Okaya, Teruo Iwasaki, Michiko Kakihana, Jiro Fujimoto, Nobuyuki Terada, Tohru Tsujimura  Journal of Hepatology  Volume 39, Issue 1, Pages (July 2003) DOI: /S (03)

2 Fig. 1 Effects of BDL on serum bilirubin and ALT in +/+ and Ws/Ws rats. (A) Total bilirubin; and (B) ALT. Rats at the age of 2 or 6 weeks underwent SHAM (S) or BDL (B), and the concentrations of serum bilirubin and the values of serum ALT were measured on day 4 after operations. *P<0.05, significant difference by the corrected Mann–Whitney tests. Journal of Hepatology  , 86-92DOI: ( /S (03) )

3 Fig. 2 Liver sections of +/+ and Ws/Ws rats that underwent BDL. (A, B) BDL-2-week-old +/+ rat; (C, D) BDL-2-week-old Ws/Ws rat; (E, F) BDL-6-week-old +/+ rat; and (G, H) BDL-6-week-old Ws/Ws rat. Sections (A, C, E, and G) were stained with hematoxylin-eosin and their adjacent sections (B, D, F, and H) were immunostained with CK-19 antibody (original magnification×160). Journal of Hepatology  , 86-92DOI: ( /S (03) )

4 Fig. 3 Numbers of CK-19 positive cells in the portal field of the livers of +/+ and Ws/Ws rats. Rats at the age of 2 or 6 weeks underwent SHAM (S) or BDL (B), and numbers of CK-19 positive cells per portal field (0.5×0.5 mm2) were counted on day 4 after operations. Bars indicate means±SE. *P<0.05, significant difference by unpaired, two-tailed Student's t-test. Journal of Hepatology  , 86-92DOI: ( /S (03) )

5 Fig. 4 Ki-67 labeling indices of BEC of +/+ and Ws/Ws rats. Rats at the age of 2 or 6 weeks underwent SHAM (S) or BDL (B), and Ki-67 labeling indices were examined on day 4 after operations. Bars indicate means±SE. *P<0.05, significant difference by unpaired, two-tailed Student's t-test. Journal of Hepatology  , 86-92DOI: ( /S (03) )

6 Fig. 5 Expression of KIT in BEC of BDL-2-week-old rats. (A) BDL-2-week-old +/+ rat; and (B) BDL-2-week-old Ws/Ws rat. Each liver section of 2-week-old +/+ and Ws/Ws rats that underwent BDL was immunostained with KIT antibody. Arrows indicate BEC immunoreacted with KIT antibody (original magnification×183). Journal of Hepatology  , 86-92DOI: ( /S (03) )


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