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Amir Abolhoda, Sumei Yu, J

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Presentation on theme: "Amir Abolhoda, Sumei Yu, J"— Presentation transcript:

1 No-React Detoxification Process: A Superior Anticalcification Method for Bioprostheses1 
Amir Abolhoda, Sumei Yu, J.Rodrigo Oyarzun, Keith R Allen, John R McCormick, Shenggao Han, Francis W Kemp, John D Bogden, Qi Lu, Shlomo Gabbay  The Annals of Thoracic Surgery  Volume 62, Issue 6, Pages (December 1996) DOI: /S (96)

2 Fig. 1 Trend of calcification of subcutaneous porcine cusp implants over a 14-week period. The glutaraldehyde-treated cusps show a progressive calcification over time. The No-React-treated cusps undergo minimal calcification with no progression over time (p <0.01). The Annals of Thoracic Surgery  , DOI: ( /S (96) )

3 Fig. 2 Light photomicrographs of glutaraldehyde-treated cusp explants at 6 weeks. (A) Disruption of the collagen fibrils and inflammatory reaction (hematoxylin and eosin). (B) A glutaraldehyde-treated cusp stained with von Kossa stain; calcium deposits are seen as black spots. (C) Higher magnification of an aortic cusp explant showing severe inflammatory cell infiltration. (D) Higher magnification of B hematoxylin and eosin. (Magnifications: A, ×100; B, ×100; C, ×200; D, ×200.) The Annals of Thoracic Surgery  , DOI: ( /S (96) )

4 Fig. 3 Light photomicrographs of No-React-treated cusp explants at 6 weeks. (A) Collagen matrix of the cusp is preserved; no inflammatory reaction is seen (hematoxylin and eosin). (B) Same cusp stained with von Kossa stain; no calcium deposits are seen. (C) Higher magnification of No-React-treated cusp explant with well-preserved architecture (hematoxylin and eosin). (D) Von Kossa stain of the cusp in slide C. (Magnifications: A, X100; B, X100; C, X200; D, X200.) The Annals of Thoracic Surgery  , DOI: ( /S (96) )

5 Fig. 4 Reverse light photomicrographs of cell culture plates incubated with pericardial tissue at time 0. (A) No-React-treated tissue (Ts) at the bottom of the figure; live mature fibroblasts at the top of the figure. (B) Glutaraldehyde-treated Ts at the bottom; live fibroblasts at the top. The Annals of Thoracic Surgery  , DOI: ( /S (96) )

6 Fig. 5 Reverse light photomicrographs of cell culture plates at 6 and 24 hours after tissue incubation, with addition of erythrosin B to the medium. (A) Healthy dividing cells adjacent to No-React tissue (Ts) at 6 hours. (B) Cells begin to acquire red stain (black round figures) 6 hours after incubation with glutaraldehyde-treated Ts. (C) At 24 hours, 100% of the cells are viable in presence of No-React-treated tissue. (D) At 24 hours, progressive loss of viability of the cells incubated with glutaraldehyde-treated tissue is notable, as evident in the entire field. The Annals of Thoracic Surgery  , DOI: ( /S (96) )

7 Fig. 6 Erythrosin B dye exclusion test observed at 48 hours. (A) The fibroblasts form a confluent monolayer around the No-React-treated tissue (Ts). (B) Near 100% cell death with glutaraldehyde-treated Ts incubation. The Annals of Thoracic Surgery  , DOI: ( /S (96) )

8 Fig. 7 Dye exclusion test: comparison of dead cell count between the No-React- and glutaraldehyde-treated tissues. The Annals of Thoracic Surgery  , DOI: ( /S (96) )

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