1. Nada Mohamed Ahmed , MT (ASCP)i
Mycology revision
Nada Mohamed Ahmed , MT (ASCP)i

2. Types of Culture media Type of culture media Name of the culture media
Picture
NON SELECTIVE
Sabouraud dextrose agar (SDA)
Selective media
Corn meal agar (CMA)
Brain Heart Infusion (BHI) agar
Bird seed agar
Mycosel Agar –special for dermatophytes
CHROMagar - special for Candida

3. Microscopic techniques – used to Identify of fungal morphology :
Name of techniques
Method
Picture
wet mount(LPCB)
1-Teased Preparation
gently tease the inverted side of the specimen into
the staining (LPAB) fluid.
2-Scotch tape preparation
touch it to the surface of the colony put it on a slide that
contains a drop of lactophenol cotton blue like,
3-Slide Culture
Cut the plug; put it on a sterile slide on top of the sterile water agar...

4. DERMATOPHYTOSIS (infection of dermatophytes)
Location
picture
Tinea corporis
- small lesions occurring anywhere on the body
Tinea Pedis
– Athlete’s Foot Infection
Tinea unguium (onychomycosis)
- nails
Tinea capitis
- head
Tinea barbae -
ringworm of the bearded areas of the face and neck.

5. Diagnosis of dermatophytes (depend on Macroconidia shape)
Shape of Macroconidia
picture
Trichophyton
Cylinder or pencil
Microsporum
Spindle
Epidermophyton
Club

6. Types of Superficial Mycoses
Causative agent
diagnosis
picture
1-PITYRIASIS VERSICOLOR
Malassezia furfur
(Lipophilic yeast)
KOH- Spaghetti and meatballs
2-TINEA NIGRA
Exophiala werneckii
Produce melanin black or brown color
Skin scrapings with KOH
Septate hyphae and yeast
3-BLACK PIEDRA
Piedraia hortae
Septate pigmented hyphae, and asci; unicellular and fusiform ascospores.
4-WHITE PIEDRA
Trichosporon.
septate hyphae that develops into arthroconidia

7. Other Mycosis: Mycosis(infection) Causative agent diagnosis picture
Subcutaneous Mycosis
Madurella mycetomatis
Madurella grisea
Demonstration of granules(graines) in the infected tissue.
Systemic Mycosis
Histoplasma capsulatum
Giemsa or Wright stains. - Small, oval yeast like cells, 2-4µ within mononuclear or polymorphonuclear cells, narrow neck
budding.
Chest radiograph
LPCB - White cottony mycelia with large (8-20µ) thick walled, spherical spores with tubercles or finger
like projections –
Tuberculate Macroconidia.

8. Candidiasis
Candida. albicans
KOH wet mount
Fungal culture
Aspergillosis
Aspergillus fumigatus
High level of IgE and IgG against aspergillus.
Increased level of eosinophils.
Sputum examination and culturePositive circulating cell wall galactomannans (are polysaccharides )
Chest X-ray.