1. Nelson, PT_Suppl_Fig2 A B

2. * ** ** Lysate Co-IP after transfections Nelson, PT_Suppl_Fig3 GRN GRN
BACE1
BACE1
n=3 biological replicates each
MiR-107 v NEG-CTRL *-p<0.01; **-p<0.0001

3. A B C D Anti-AGO co-IP with downstream qPCR
Nelson, PT_Suppl_Fig4 A
miR-107*
c c c u u c u a
ucu ugcuuu agcu cu uacaguguugc uug ggc u
||| |||||| |||| || ||||||||||| ||| ||| g
aga acgaaa ucgg ga auguuacgacg aac uug g
c cua c a
miR 5’ 3’
Homo sapiens miR-107 stem-loop B
u u c
agcu cu uacaguguugc uug miR-107*
|||| || ||||||||||| |
ucgg ga auguuacgacg a miR-107
acua c 5’ 3’ 5’ 3’
miR-107 duplex for transfection C D
Anti-AGO co-IP with downstream qPCR

4. A B Y axis: # miR-16 seed sequences/1000 nts in 5’utr, CDS, and 3’UTR
Nelson, PT_Suppl_Fig5
Y axis: # miR-16 seed sequences/1000 nts
in 5’utr, CDS, and 3’UTR A
X axis:
miR-16 targets
sorted by
enrichment
in AGO co-IP
(50 mRNAs/grp) B
X axis:
miR-16 targets
sorted by
mRNA lysate
“knock-down”
(50 mRNAs/grp)