Presentation is loading. Please wait.

Presentation is loading. Please wait.

Intraoperative transfection of vein grafts with the NFκB decoy in a canine aortocoronary bypass model: a strategy to attenuate intimal hyperplasia  Takuji.

Published byΚαλλιόπη Βαρνακιώτης Modified over 5 years ago

Similar presentations

Presentation on theme: "Intraoperative transfection of vein grafts with the NFκB decoy in a canine aortocoronary bypass model: a strategy to attenuate intimal hyperplasia  Takuji."— Presentation transcript:

1 Intraoperative transfection of vein grafts with the NFκB decoy in a canine aortocoronary bypass model: a strategy to attenuate intimal hyperplasia  Takuji Shintani, MD, Yoshiki Sawa, MD, Toshiki Takahashi, MD, Goro Matsumiya, MD, Nariaki Matsuura, MD, Yuji Miyamoto, MD, Hikaru Matsuda, MD  The Annals of Thoracic Surgery  Volume 74, Issue 4, Pages (October 2002) DOI: /S (02)

2 Fig 1 Localization of fluorescent isothiocyanate (FITC)-labeled oligodeoxynucleotides (ODNs). Concentrated green FITC-labeled ODN signals are indicated in this fluorescence photomicrograph. ODNs are seen in the nuclei of the media and intima. Arrows indicate FITC-labeled ODNs in the nuclei of the media (×100 magnification). The numbers of FITC-positive and hematoxylin-positive nuclei were counted by using the public-domain image processing and analysis program (image, × 100 magnification). The average transfection efficiency of the decoy on the cross-section, defined as the ratio of the number of FITC-positive nuclei to the total number of nuclei based on hematoxylin staining, was 77% ± 20%. The Annals of Thoracic Surgery  , DOI: ( /S (02) )

3 Fig 2 Cross-sections of the middle portion of vein grafts.Arrows indicate the internal elastic lamina. The medial and intimal areas were calculated by using the computerized image analysis program, image. In comparison with that of the scrambled decoy group (a), neointimal hyperplasia of the NFκB decoy group (b) was significantly suppressed. (Hematoxylin & eosin staining, ×40 magnification.) (I= intima;M= media.) The Annals of Thoracic Surgery  , DOI: ( /S (02) )

4 Fig 3 α-actin staining.Arrows indicate the internal elastic lamina. α-actin was stained brown immunohistochemically by using the immunoperoxidase avidin-biotin complex system with nickel chloride color modification. Transfection of NFκB decoy tended to suppress the proliferation of the smooth muscle cells in the media. (a) Scrambled decoy group. (b) NFκB decoy group. (×200 magnification.) (I = intima;M = media.) The Annals of Thoracic Surgery  , DOI: ( /S (02) )

5 Fig 4 Extracellular matrix staining. Arrows indicate the internal elastic lamina. In Masson-trichrome staining, the extracellular matrix is stained blue. (a) Scrambled decoy group. In the (b) NFκB decoy group, suppression of superfluous synthesis of the extracellular matrix in the neointima was seen. (×100 magnification.) (I = intima;M= media.) The Annals of Thoracic Surgery  , DOI: ( /S (02) )

Download ppt "Intraoperative transfection of vein grafts with the NFκB decoy in a canine aortocoronary bypass model: a strategy to attenuate intimal hyperplasia  Takuji."

Similar presentations

Alpha-lipoic acid inhibits fractalkine expression and prevents neointimal hyperplasia after balloon injury in rat carotid artery  Kyeong-Min Lee, Keun-Gyu.

Alpha-lipoic acid inhibits fractalkine expression and prevents neointimal hyperplasia after balloon injury in rat carotid artery  Kyeong-Min Lee, Keun-Gyu.
The Role of ex-vivo Gene Therapy of Vein Grafts with Egr-1 Decoy in the Suppression of Intimal Hyperplasia  M. Peroulis, J. Kakisis, A. Kapelouzou, A.

The Role of ex-vivo Gene Therapy of Vein Grafts with Egr-1 Decoy in the Suppression of Intimal Hyperplasia  M. Peroulis, J. Kakisis, A. Kapelouzou, A.
Wound healing around and within saphenous vein bypass grafts

Wound healing around and within saphenous vein bypass grafts
Biodegradable polymer with collagen microsponge serves as a new bioengineered cardiovascular prosthesis  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Hajime.

Biodegradable polymer with collagen microsponge serves as a new bioengineered cardiovascular prosthesis  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Hajime.
Ruptured femoropopliteal artery aneurysms in von Recklinghausen neurofibromatosis  Fabian Gutarra, MD, Javier Rodriguez Asensio, MD, Miguel Miceli, MD,

Ruptured femoropopliteal artery aneurysms in von Recklinghausen neurofibromatosis  Fabian Gutarra, MD, Javier Rodriguez Asensio, MD, Miguel Miceli, MD,
Therapeutic Effect of Midkine on Cardiac Remodeling in Infarcted Rat Hearts  Shinya Fukui, MD, Satoru Kitagawa-Sakakida, MD, PhD, Sin Kawamata, MD, PhD,

Therapeutic Effect of Midkine on Cardiac Remodeling in Infarcted Rat Hearts  Shinya Fukui, MD, Satoru Kitagawa-Sakakida, MD, PhD, Sin Kawamata, MD, PhD,
Experimental Bronchiolitis Obliterans Induced by In Vivo HVJ-Liposome–Mediated Endothelin-1 Gene Transfer  Shin-ichi Takeda, MD, Yoshiki Sawa, MD, Masato.

Experimental Bronchiolitis Obliterans Induced by In Vivo HVJ-Liposome–Mediated Endothelin-1 Gene Transfer  Shin-ichi Takeda, MD, Yoshiki Sawa, MD, Masato.
Late Complications of Gelatin-Resorcin-Formalin Glue in the Repair of Acute Type A Aortic Dissection  Hiroki Hata, MD, PhD, Hiroshi Takano, MD, PhD, Goro.

Late Complications of Gelatin-Resorcin-Formalin Glue in the Repair of Acute Type A Aortic Dissection  Hiroki Hata, MD, PhD, Hiroshi Takano, MD, PhD, Goro.
Gene transfer to vein graft wall by HVJ–liposome method: time course and localization of gene expression  Hong-zhi Bai, MD, Yoshiki Sawa, MD, Wei-da Zhang,

Gene transfer to vein graft wall by HVJ–liposome method: time course and localization of gene expression  Hong-zhi Bai, MD, Yoshiki Sawa, MD, Wei-da Zhang,
Ezetimibe reduces intimal hyperplasia in rabbit jugular vein graft

Ezetimibe reduces intimal hyperplasia in rabbit jugular vein graft
Lysyl oxidase expression in a rat model of arterial balloon injury

Lysyl oxidase expression in a rat model of arterial balloon injury
Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.
True extracranial carotid artery aneurysm in a child

True extracranial carotid artery aneurysm in a child
Histologic analysis of stent graft oversizing in the thoracic aorta

Histologic analysis of stent graft oversizing in the thoracic aorta
Novel Tissue-Engineered Biodegradable Material for Reconstruction of Vascular Wall  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Satoshi Taketani, MD, Kei Torikai,

Novel Tissue-Engineered Biodegradable Material for Reconstruction of Vascular Wall  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Satoshi Taketani, MD, Kei Torikai,
Combined Strategy Using Myoblasts and Hepatocyte Growth Factor in Dilated Cardiomyopathic Hamsters  Haruhiko Kondoh, MD, Yoshiki Sawa, MD, Norihide Fukushima,

Combined Strategy Using Myoblasts and Hepatocyte Growth Factor in Dilated Cardiomyopathic Hamsters  Haruhiko Kondoh, MD, Yoshiki Sawa, MD, Norihide Fukushima,
Reorganization of cytoskeletal proteins and prolonged life expectancy caused by hepatocyte growth factor in a hamster model of late-phase dilated cardiomyopathy 

Reorganization of cytoskeletal proteins and prolonged life expectancy caused by hepatocyte growth factor in a hamster model of late-phase dilated cardiomyopathy 
Mark Wengrovitz, MD. , Lulseged G. Selassie, MD. , Robert R. M

Mark Wengrovitz, MD. , Lulseged G. Selassie, MD. , Robert R. M
Basic fibroblast growth factor slow release stent graft for endovascular aortic aneurysm repair: A canine model experiment  Masaki Kajimoto, MD, Takatsugu.

Basic fibroblast growth factor slow release stent graft for endovascular aortic aneurysm repair: A canine model experiment  Masaki Kajimoto, MD, Takatsugu.
Renal artery stenosis and aneurysms associated with neurofibromatosis

Renal artery stenosis and aneurysms associated with neurofibromatosis

Similar presentations

Ads by Google