1. Volume 136, Issue 5, Pages 1796-1805.e6 (May 2009)
A Polymorphism in MAPKAPK3 Affects Response to Interferon Therapy for Chronic Hepatitis C 
Hironobu Tsukada, Hidenori Ochi, Toshiro Maekawa, Hiromi Abe, Yoshifumi Fujimoto, Masataka Tsuge, Hiroshi Takahashi, Hiromitsu Kumada, Naoyuki Kamatani, Yusuke Nakamura, Kazuaki Chayama 
Gastroenterology 
Volume 136, Issue 5, Pages e6 (May 2009)
DOI: /j.gastro
Copyright © 2009 AGA Institute Terms and Conditions

2. Figure 1
Allele-specific transcript quantification of MAPKAPK3. The allele-specific MAPKAPK3 mRNA expression ratio for haplotype 2 to haplotype 1 is shown. Individual data from liver biopsies from 5 patients are indicated. Each experiment was performed in triplicate assay at least 3 times. Data represent the mean ± SD.
Gastroenterology  , e6DOI: ( /j.gastro )
Copyright © 2009 AGA Institute Terms and Conditions

3. Figure 2
Effects of MAPKAPK3 on IFN-α–induced gene transcription via ISRE and GAS elements. Huh7 cells were transfected with both 1 ng renilla luciferase expression vector pRL-TK (internal control) and 10 ng firefly luciferase expression vector (A) pISRE-TA-Luc or (B) pGAS-TA-Luc, in conjunction with 40 ng expression plasmid pDEST51/mock (negative control, open bar), pDEST51/suppressor of cytokine signaling 1 (socs1) (positive control, black bar), or pDEST51/MAPKAPK3 (gray bar). After 24 hours, cells were stimulated with IFN-alfa for another 20 hours, and luciferase induction was measured. Firefly luciferase activity was normalized by renilla luciferase activity. Data represent the mean ± SD of triplicate assay. *P < .05 for comparison with mock.
Gastroenterology  , e6DOI: ( /j.gastro )
Copyright © 2009 AGA Institute Terms and Conditions