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Sedimentation method for preparation of postoperatively salvaged unwashed shed blood in orthopaedic surgery  M Muñoz, S García-Segovia, D Ariza, A Cobos,

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Presentation on theme: "Sedimentation method for preparation of postoperatively salvaged unwashed shed blood in orthopaedic surgery  M Muñoz, S García-Segovia, D Ariza, A Cobos,"— Presentation transcript:

1 Sedimentation method for preparation of postoperatively salvaged unwashed shed blood in orthopaedic surgery  M Muñoz, S García-Segovia, D Ariza, A Cobos, J.A. García-Erce, D Thomas  British Journal of Anaesthesia  Volume 105, Issue 4, Pages (October 2010) DOI: /bja/aeq174 Copyright © 2010 The Author(s) Terms and Conditions

2 Fig 1 Blood processing with a colloid solution. (a) Colloid solution (HES) was added to diluted venous blood to a final concentration of 15%, 30%, or 45% (colloid solution volume/total volume×100), and an aliquot of the mix was obtained. (b) Thereafter, the syringes were set upright and left for 20 or 30 min at room temperature to allow for RBC sedimentation. (c) Then, the supernatant was evacuated from the syringe and the remaining content shaken and analysed. C, control, no colloid added (left); HES, hydroxyethyl starch 450 kDa 30% (right). British Journal of Anaesthesia  , DOI: ( /bja/aeq174) Copyright © 2010 The Author(s) Terms and Conditions

3 Fig 2 Recovery of RBCs and removal of leucocytes (WBC), platelets, plasma, total protein, and PFHB after processing postoperative USB by the addition of different colloid solutions to a final concentration of 15% (HES-450-1) or 30% (HES-200, HES-450-2, and gelatine) (colloid solution volume/total volume×100) and incubation for 30 min, according to the procedure described in Figure 1. Each colloid concentration was tested in 10 separate experiments. British Journal of Anaesthesia  , DOI: ( /bja/aeq174) Copyright © 2010 The Author(s) Terms and Conditions

4 Fig 3 Removal of IL-6, IL-8, TNF-α, GOT, CK, and LDH after processing postoperative USB by the addition of different colloid solutions to a final concentration of 15% (HES-450-1) or 30% (HES-200, HES-450-2, gelatine) (colloid solution volume/total volume×100) and incubation for 30 min, according to the procedure described in Figure 1. Each colloid concentration was tested in 10 separate experiments. British Journal of Anaesthesia  , DOI: ( /bja/aeq174) Copyright © 2010 The Author(s) Terms and Conditions


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