1. Prolonged 24-hour subzero preservation of heterotopically transplanted rat hearts using antifreeze proteins derived from arctic fish 
Gabriel Amir, MD, Boris Rubinsky, PhD, Liana Horowitz, MS, Liron Miller, Jonathan Leor, MD, Yigal Kassif, MD, David Mishaly, MD, Aram K Smolinsky, MD, Jacob Lavee, MD 
The Annals of Thoracic Surgery 
Volume 77, Issue 5, Pages (May 2004)
DOI: /j.athoracsur

2. Fig 1
Viability scores of hearts preserved 12, 18, and 24 hours at 4°C in University of Wisconsin (UW) solution and at −1.3°C in UW solution with antifreeze protein (AFP) III 30 minutes after reperfusion. Values are presented as medians for descriptive purposes. Viability scores were significantly better for hearts preserved 18 and 24 hours at −1.3°C compared with hearts preserved at 4°C (p < 0.05). (NS = not significant.)
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )

3. Fig 2
Viability scores of hearts preserved 12, 18, and 24 hours at 4°C in University of Wisconsin (UW) solution and at −1.3°C in UW solution with antifreeze protein (AFP) III 60 minutes after reperfusion. Values are presented as medians for descriptive purposes. Viability scores were significantly better for hearts preserved 18 and 24 hours at −1.3°C compared with hearts preserved at 4°C (p < 0.05). (NS = not significant.)
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )

4. Fig 3
(A: ×200 magnification; B: ×400 magnification) Representative hematoxylin and eosin stainings of heterotopically transplanted rat hearts that were preserved for 12 hours at −1.3°C in University of Wisconsin solution with antifreeze protein III. Cardiomyocyte structure is preserved; there is no evidence of necrosis. (C: ×200 magnification; D: ×400 magnification) Representative hematoxylin & eosin stainings of heterotopically transplanted rat hearts that were preserved for 12 hours at −1.3°C in University of Wisconsin solution without antifreeze protein III and froze. Cardiomyocyte structural damage is evident; there is loss of myocardial striations, and large areas of necrosis are visible.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )

5. Fig 4
(A–C) Representative slides of heterotopically transplanted rat hearts preserved for 12 hours at 4°C. (D: ×5,000; E: ×10,000) Representative slides of heterotopically transplanted rat hearts preserved for 12 hours at −1.3°C using antifreeze protein III. (F: ×5,000; G: ×10,000) Representative slides of heterotopically transplanted rat hearts that were preserved for 12 hours at −1.3°C in University of Wisconsin solution without antifreeze protein III and froze.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )