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Efficacy of fragmin/protamine microparticles containing fibroblast growth factor-2 (F/P MPs/FGF-2) to induce collateral vessels in a rabbit model of hindlimb.

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Presentation on theme: "Efficacy of fragmin/protamine microparticles containing fibroblast growth factor-2 (F/P MPs/FGF-2) to induce collateral vessels in a rabbit model of hindlimb."— Presentation transcript:

1 Efficacy of fragmin/protamine microparticles containing fibroblast growth factor-2 (F/P MPs/FGF-2) to induce collateral vessels in a rabbit model of hindlimb ischemia  Takuya Horio, MD, Masanori Fujita, MD, PhD, Yoshihiro Tanaka, MD, Masayuki Ishihara, PhD, DEng, Satoko Kishimoto, VMD, PhD, Shingo Nakamura, PhD, Kazuo Hase, MD, PhD, Tadaaki Maehara, MD, PhD  Journal of Vascular Surgery  Volume 54, Issue 3, Pages (September 2011) DOI: /j.jvs Copyright © 2011 Society for Vascular Surgery Terms and Conditions

2 Fig 1 The rabbit acute hindlimb ischemia model was created by resecting the left femoral artery from the inguinal ligament to the popliteal fossa and bifurcation of the saphenous artery. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2011 Society for Vascular Surgery Terms and Conditions

3 Fig 2 Laser Doppler perfusion imaging. No significant differences in blood flow ratio of the control (white bar), FGF-2-treated (light gray bar), F/P MPs-treated (dark gray bar), and F/P MPs/FGF-2-treated (black bar) groups were observed on day 0. The blood flow ratio showed significant improvement in the F/P MPs-treated and F/P MPs/FGF-2-treated groups on days 14 and 28. Data represent the mean ± SD of six determinations. *P < .05. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2011 Society for Vascular Surgery Terms and Conditions

4 Fig 3 Measurement of calf blood pressure. While calf blood pressure was not detected in the ischemic limbs of the four groups before the initiation of therapy (day 0), they showed significant improvement in the F/P MPs/FGF-2-treated (black bars) group on days 14 and 28 in comparison to the control (white bars), FGF-2-treated (light gray bars), and F/P MPs-treated groups (dark gray bars). Data represent the mean ± SD of six determinations. *P < .01. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2011 Society for Vascular Surgery Terms and Conditions

5 Fig 4 Angiograms of ischemic limb on day 28. A, control group. B, FGF-2-treated group. C, F/P MPs-treated group. D, F/P MPs/FGF-2-treated group. The top angiogram shows a representative for intact limb. Photographs are representative of six angiograms in each of four groups. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2011 Society for Vascular Surgery Terms and Conditions

6 Fig 5 Quantification of visible collateral arteries under stereoscopic viewing on day 28. Data show the number of angiographically visible collateral arteries in the control group (white bar), FGF-2-treated (light gray bar), F/P MPs-treated (dark gray bar), and F/P MPs/FGF-2-treated (black bar) groups. Data represent the mean ± SD of six determinations. *P < .01,**P <.001, NS, not significant. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2011 Society for Vascular Surgery Terms and Conditions

7 Fig 6 CD31 and α-SM actin double-immunostaining histology. The sections were double-immunostained for CD-31 and α-SM actin to detect vascular smooth muscle cells upon the same field images stained with Masson's trichrome reagent. A, control group. B, FGF-2-treated group. C, F/P MPs-treated group. D, F/P MPs/FGF-2-treated group. Each photograph is a representative of six histologic samples in each group. Black arrows indicate visible blood vessels, and square areas immunostained with α-SM actin alone were magnified in each photograph. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2011 Society for Vascular Surgery Terms and Conditions

8 Fig 7 The number of capillaries was counted from 10 different fields randomly selected at ×200 magnification in a blinded manner determined by CD31 and α-SM actin double-immunostaining superimposed upon the same field images stained with Masson's trichrome reagent. Data represent the mean ± SD of six determinations. *P < .05, **P < .01. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2011 Society for Vascular Surgery Terms and Conditions

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