1. Electron microscopy confirms the exterior location of GFP on chimeric GFPcore particles.
Electron microscopy confirms the exterior location of GFP on chimeric GFPcore particles. (A) Cryoelectron micrograph. An aliquot from the particle fraction shows abundant particles in two size-classes. (B and C) Three-dimensional image reconstructions of the large (T = 4) GFPcore particles at 2-nm resolution. The surface view in B reveals a double-layered shell with the inner layer strongly resembling authentic core particles. The outer layer therefore most likely represents partially ordered GFP. The double-shell architecture is emphasized in the equatorial cross section in C. White areas correspond to high electron density.
Peter A. Kratz et al. PNAS 1999;96:5:
©1999 by National Academy of Sciences