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Occupational endotoxin exposure and a novel luminol-enhanced chemiluminescence assay of nasal lavage neutrophil activation  Romy Seth, Marcos Ribeiro,

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Presentation on theme: "Occupational endotoxin exposure and a novel luminol-enhanced chemiluminescence assay of nasal lavage neutrophil activation  Romy Seth, Marcos Ribeiro,"— Presentation transcript:

1 Occupational endotoxin exposure and a novel luminol-enhanced chemiluminescence assay of nasal lavage neutrophil activation  Romy Seth, Marcos Ribeiro, MD, Alex Romaschin, PhD, Jeremy A. Scott, PhD, Michael Manno, MSc, James A. Scott, PhD, Gary M. Liss, MD, Susan M. Tarlo, MBBS  Journal of Allergy and Clinical Immunology  Volume 127, Issue 1, Pages (January 2011) DOI: /j.jaci Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Chemiluminescence in nasal lavage fluid (NALF) samples. Zymosan binds activated CR3 receptors on a polymorphonuclear neutrophil (PMN), resulting in a respiratory burst releasing reactive oxygen species. Oxygen radicals then combine with luminol, causing light release, which is measured with a chemiluminometer. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Median NAL neutrophil activation in control subjects (A) and ACWs (B) obtained before and after exposures. NAL neutrophil activation did not change between the pre-exposure and postexposure periods, but results were significantly different between control subjects and ACWs. ∗P = .006 and ∗∗∗P = versus control subjects at the same time point. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions


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