1. Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis
Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis ARepresentative images of the hematoxylin–eosin staining of liver sections obtained from animals fed a chow or a HFD and administered with either AAV8‐hAAT‐null or 5 × 1010 vg/mouse AAV8‐hAAT‐FGF21 vectors. HFD clearly induced the deposition of lipid droplets in the liver, and this was reverted by AAV8‐hAAT‐FGF21 treatment both in young adults and in adults. Scale bars: 100 μm.B, CFed hepatic triglyceride and cholesterol content in the same cohorts of animals.DImmunostaining for the macrophage‐specific marker Mac2 of liver sections from animals fed a HFD that received either AAV8‐hAAT‐null or 5 × 1010 vg/mouse AAV8‐hAAT‐FGF21 vectors. Red arrows indicate the presence of crown‐like structures. Scale bars: 200 μm and 50 μm (inset).E, FQuantification by qRT–PCR of the expression of collagen 1 in the liver in the group of animals that initiated the HFD feeding and received FGF21 vectors as young adults (E) or adults (F).Data information: All values are expressed as mean ± SEM. In (B, C, E, F), young adults: AAV8‐hAAT‐null chow (n = 10 animals), AAV8‐hAAT‐null HFD (n = 8), AAV8‐hAAT‐FGF21 HFD 5 × 1010 vg (n = 8). Adults: AAV8‐hAAT‐null chow (n = 7), AAV8‐hAAT‐null HFD (n = 7), AAV8‐hAAT‐FGF21 HFD 5 × 1010 vg (n = 7). In (B, C, E, F), data were analyzed by one‐way ANOVA with Tukey's post hoc correction. *P < 0.05, **P < 0.01, and ***P < 0.001 versus the chow‐fed null‐injected group. #P < 0.05, ##P < 0.01, and ###P < 0.001 versus the HFD‐fed null‐injected group. HFD, high‐fat diet.
Veronica Jimenez et al. EMBO Mol Med. 2018;10:e8791
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