1. Control and Prevention of emerging and future pathogens at cellular and molecular level throughout the food chain
Prof. Mogens Jakobsen
Dept. of Food Science
The Royal Veterinary and Agricultural University, Copenhagen, Denmark
Coordinator of PathogenCombat

2. PathogenCombat Objective
To provide new essential information and methods to the food industry and public authorities on how to reduce the prevalence of new and re-emerging foodborne pathogens
Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald.

3. Pathogens
Bacteria
Gram+: Listeria monocytogenes, Mycobacterium avium subsp. paratuberculosis
Gram-: Campylobacter jejuni & shiga-like toxin producing Escherichia coli (STEC)
Yeast
Invasive variants of Saccharomyces cerevisiae
Fungi
An ochratoxin A producing filamentous fungus (Penicillium nordicum)
Viruses
Hepatitis E & tickborne encephalitis virus
Heat stable bacterial toxins
Staphylococcus aureus

4. HIGH TECHNOLOGY PLATFORMS
Pathogen matrix interactions
Bioimaging, Flourescent Ratio Imaging Microscopy (FRIM), Force Scope, Confocal Laser Scanning Microscopy (CLSM), Atomic Force Microscopy (AFM)
Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald.

5. HIGH TECHNOLOGY PLATFORMS
Host-pathogen interaction
Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald.
Mamalian functional cell models to replace experimental animals

6. HIGH TECHNOLOGY PLATFORMS
Detection and monitoring
Functional genomic and bioinformatic based development of DNA-microarrays for pathogen detection viability/stress response/virulence
Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald.

7. HIGH TECHNOLOGY PLATFORMS
Robotic system
High Throughput Screening (HTS) for thousands of protective and probiotic cultures
Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald.

8. HIGH TECHNOLOGY PLATFORMS
Modelling microbial population behaviour throughout the food chain
Identification of CCP by use of stochastic simulation models based upon quantification of numbers of pathogens including mycotoxin producing fungi and their virulence/toxcicity expression along the entire food chain
Estimation of microbial food safety risk at time of consumption based upon stochastic simulation modelling of number of pathogens and their virulence/toxcicity expression
Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald.

9. PathogenCombat Deliverable I + II
New approach to estimate the prevalence of virulent pathogens up to the time of consumption
New methods to control the viability and virulence of pathogens throughout the food chain until the time of consumption

10. FRIM

11. FRIM - Simultaneous detection of physiological status by intracellular pH-determination
Listeria monocytogenes

12. Force scope – quantifying the microbial adhesion by a novel technique

13. PathogenCombat Deliverable III
New method for estimation of host-pathogen interaction based upon functional cell models

14. Novel processing technologies to inactivate pathogens
PathogenCombat Deliverable IV
Novel processing technologies to inactivate pathogens
New probiotic and protective cultures to eliminate pathogens
Elimination of biofilms based upon hygienic design

15. New mathematical models covering food at time of consumption
PathogenCombat Deliverable V - VII
Established strategies and actions for disseminating project deliverables for food SMEs
New mathematical models covering food at time of consumption
New cost effective food safety
management systems

16. PathogenCombat Deliverable VIII
Improved awareness of food safety for the European consumer, food industries and regulatory agencies

18. PathogenCombat 5 year Integrated Project RTD I RTD II RTD III RTD IV
Discovery and Development
Detection &
Virulence
traits
Control &
Prevention
Application and Management
NTC I
SME network
NTC II
Training activities
NTC III
Consumer awareness, food industry and regulatory agencies
Total budget 14.3 Mio €
EU grant to the budget 11,3 Mio €

19. Training
Training of SME food producing partners and implementation of project deliverables
Workshop for about 150 European, INCO and Accession Country SMEs and regulatory agencies
Pan European dissemination of deliverables via generic food safety management system
Exchange of scientists ( INCO/Accession Countries)