1. Fingerprints of anergic T cells
Oskar Lechner, Jörg Lauber, Anke Franzke, Adelaida Sarukhan, Harald von Boehmer, Jan Buer 
Current Biology 
Volume 11, Issue 8, Pages (April 2001)
DOI: /S (01)

2. Figure 1
(a) Genes differentially regulated in anergic versus naive CD4+ T cells. Total RNA of in vivo-anergized T cells from TCR × Ig-HA and naive T cells from TCR-HA mice, respectively, were used as templates to generate specific 32P-labeled cDNA probes for expression array analysis (Clontech). RT probes were hybridized to individual cDNA blots overnight and washed, and gene expression was determined by exposing the arrays to Phosphor Imaging screens (Fuji) and analyzed using a Fuji Bas bit image analysis system. Expression levels were determined by measuring the spot density for each gene using Array-Vision software (Version 5.1). To compare density values among the two cell populations, the level of each gene was normalized to the total of all genes measured (Table 1). One out of two independent array analyses results are shown. (b) Genes differentially regulated in anergic versus activated CD4+ T cells. Total RNA of in vivo-anergized T cells from TCR × Ig-HA mice and naive T cells from Rag-2−/− TCR-HA mice, respectively, were used as templates to generate 32P-labeled cDNA probes for expression array analysis (Clontech). For further details see Figure 1
Current Biology  , DOI: ( /S (01) )

3. Figure 1
(a) Genes differentially regulated in anergic versus naive CD4+ T cells. Total RNA of in vivo-anergized T cells from TCR × Ig-HA and naive T cells from TCR-HA mice, respectively, were used as templates to generate specific 32P-labeled cDNA probes for expression array analysis (Clontech). RT probes were hybridized to individual cDNA blots overnight and washed, and gene expression was determined by exposing the arrays to Phosphor Imaging screens (Fuji) and analyzed using a Fuji Bas bit image analysis system. Expression levels were determined by measuring the spot density for each gene using Array-Vision software (Version 5.1). To compare density values among the two cell populations, the level of each gene was normalized to the total of all genes measured (Table 1). One out of two independent array analyses results are shown. (b) Genes differentially regulated in anergic versus activated CD4+ T cells. Total RNA of in vivo-anergized T cells from TCR × Ig-HA mice and naive T cells from Rag-2−/− TCR-HA mice, respectively, were used as templates to generate 32P-labeled cDNA probes for expression array analysis (Clontech). For further details see Figure 1
Current Biology  , DOI: ( /S (01) )

4. Figure 2
FACS analysis of intracellular CTLA-4 and surface PD-1 on in vivo naive, anergic, and in vitro anti-CD3-activated TCR-HA T cells. Histograms were obtained by gating on CD T cells. Broken lines represent background staining (I.C. indicates intracellular, and E.C. indicates extracellular)
Current Biology  , DOI: ( /S (01) )

5. Figure 3
(a) Interrelationships between differentially regulated genes at the level of TCR signal transduction, regulation of transcription, and cell fate in anergic CD4+ T cells relative to naive or resting cells. (b) Interrelationships between differentially regulated genes at the level of cytokine signaling and proliferation in anergic CD4+ T cells relative to naive or resting cells
Current Biology  , DOI: ( /S (01) )

6. Figure 3
(a) Interrelationships between differentially regulated genes at the level of TCR signal transduction, regulation of transcription, and cell fate in anergic CD4+ T cells relative to naive or resting cells. (b) Interrelationships between differentially regulated genes at the level of cytokine signaling and proliferation in anergic CD4+ T cells relative to naive or resting cells
Current Biology  , DOI: ( /S (01) )