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How far can we simplify in vitro diagnostics for grass pollen allergy

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1 How far can we simplify in vitro diagnostics for grass pollen allergy
How far can we simplify in vitro diagnostics for grass pollen allergy?: A study with 17 whole pollen extracts and purified natural and recombinant major allergens  Ronald van Ree, PhD, W.Astrid van Leeuwen, Rob C. Aalberse, PhD  Journal of Allergy and Clinical Immunology  Volume 102, Issue 2, Pages (August 1998) DOI: /S (98) Copyright © 1998 Mosby, Inc. Terms and Conditions

2 Fig. 1 High exposure to S. cereale (Sec c) pollen. RAST values (n = 63) for S. cereale pollen are plotted against those found for D. glomerata (Dac g) pollen. Results are expressed in RU/ml. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

3 Fig. 2 High exposure to C. dactylon (cyn d) pollen. RAST values (n = 77) for C. dactylon are pollen are plotted against those found for Po. Pratensis (Poa P) and D. glomerata pollen. Results are expressed in RU/ml. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

4 Fig. 3 C. dactylon (Cyn D) RAST inhibition. C. dactylon RAST was inhibited with C. dactylon, D. glomerata (Dac g), and Po. pratensis (Poa p). RAST values of two sera for C. dactylon, D. glomerata, and Po. pratensis, respectively, were 157.8, 162.1, and RU/ml (A) and 5.5, 5.3, and 5.9 RU/ml (B). Results are expressed as percent of uninhibited value. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

5 Fig. 4 RAST with purified group 1 and 5 allergens A, RAST results (n = 154) for natural Lol p 1 and Lol p 5 were combined and plotted against RAST for L. perenne (Lol p) extract. B, RAST results (n = 141) for recombinant (rec) Phl p 1 and Phl p 5 were combined and plotted against RAST for L. perenne extract. Results are expressed in RU/ml. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

6 Fig. 5 Lol p 1 RAST inhibition. Lol p 1 RAST was inhibited with rPhl p 1 (rec) and with Phl. pratense extract (nat) for three sera (#1, #2, and #3). Results are expressed as percent of uninhibited value. Uninhibited RAST values were 7.1, 5.5, and 6.3 RU/ml, respectively (serum dilution, 1:5). Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

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