1. From Nano to Macro: Multiscale Materials for Improved Stem Cell Culturing and Analysis 
Jeroen Leijten, Ali Khademhosseini 
Cell Stem Cell 
Volume 18, Issue 1, Pages (January 2016)
DOI: /j.stem
Copyright © 2016 Elsevier Inc. Terms and Conditions

2. Figure 1
Schematic Depiction of Nanoscale, Microscale, and Macroscale Interactions, which Are Temporally Regulated
Cell Stem Cell  , 20-24DOI: ( /j.stem )
Copyright © 2016 Elsevier Inc. Terms and Conditions

3. Figure 2 Multiscale Biomaterials to Control Stem Cell Behavior
(A) Integrating technologies and approaches for optimizing desired features across a range of length scales, while providing temporal control of selected parameters, will generate robust multiscale materials to improve stem cell culture platforms. High-throughput analyses to study how encoded features perturb cellular environments will enable systematic and rigorous investigations into stem cell behavior and facilitate iterative design of biomimetic materials. Specifically, cellular behavior can be guided using (B) temporal control, e.g., light-triggered expression of adhesion moieties, e.g., RGDs, to recruit cell populations such as neutrophils (green) and macrophages (red) in a temporal manner, which can control the formation of the fibrous capsule in vivo; (C) microscale control, e.g., micropatterning to harness the microtopography and cell placement in co-cultures to enhance the function of assembled microtissues such as albumin production by iPSC-derived hepatocyte-like cells (red) and stromal cells (green); (D) macroscale control, e.g., 3D printing to recreate organ shape and tissue function such as embryonic hearts with a complex internal trabecular structure; and (E) nanoscale control, e.g., nanotopographies to steer stem cell behavior such as the osteogenic differentiation of human mesenchymal stem cells on grooved electrospun fibers. Reproduced with permission (Gandavarapu et al., 2014; Hinton et al., 2015; Klein et al., 2015; Lee et al., 2015; Nandakumar et al., 2013; Stevens et al., 2013). Reprinted with permission from AAAS.
Cell Stem Cell  , 20-24DOI: ( /j.stem )
Copyright © 2016 Elsevier Inc. Terms and Conditions