1. Effect of Zc3h10 overexpression in myoblasts and myotubes
Effect of Zc3h10 overexpression in myoblasts and myotubes mtDNA levels, Tfam and myogenesis markers MyoD, MyHC7, TnnI1, and Mef2c RNA expression levels during C2C12 differentiation. n = 3. Statistical analysis was performed by one‐way ANOVA with Dunnet post‐test. *P < 0.01, **P < 0.01, ***P < vs. day −1.Scheme of the experimental plan followed up for Zc3h10 overexpression with adenoviral vectors in proliferating myoblasts.Zc3h10 expression levels in control and Flag‐Zc3h10 myotubes at 24, 48, and 72 h from differentiation. n = 3. Statistical analysis was performed by Student's t‐test. *P < 0.05, **P < 0.01, ***P < vs. control.Western blot to detect Flag‐tagged Zc3h10 at 24, 48, and 72 h after adenoviral overexpression in differentiated myotubes.mRNA expression levels of mitochondria and myogenesis markers in Zc3h10 overexpressing C2C12 myotubes 48 h after induction of differentiation. n = 3. Statistical analysis was performed by Student's t‐test. *P < 0.05, **P < 0.01, ***P < vs. control.C2C12 myotube fusion index 72 h after induction of differentiation with relative quantification and C2C12 myoblast proliferation rate of control and flag‐Zc3h10 cells. Fusion index, n = 4. Student's t‐test, ***P < vs. control. Proliferation, n = 3. Statistical analysis was performed by two‐way ANOVA with Sidak post‐test vs. control.Data information: n indicates the number of biological replicates. Data are expressed as mean ± SD, and P‐values were calculated from the indicated n independent experiments according to [44].
Matteo Audano et al. EMBO Rep. 2018;19:e45531
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