Presentation is loading. Please wait.

Presentation is loading. Please wait.

Generation of adult human T-cell progenitors for immunotherapeutic applications  Laura Simons, MD, PhD, Kuiying Ma, MSc, Corinne de Chappedelaine, PhD,

Published byAnders Thomsen Modified over 5 years ago

Similar presentations

Presentation on theme: "Generation of adult human T-cell progenitors for immunotherapeutic applications  Laura Simons, MD, PhD, Kuiying Ma, MSc, Corinne de Chappedelaine, PhD,"— Presentation transcript:

1 Generation of adult human T-cell progenitors for immunotherapeutic applications 
Laura Simons, MD, PhD, Kuiying Ma, MSc, Corinne de Chappedelaine, PhD, Ranjita Devi Moiranghtem, PhD, Elodie Elkaim, MD, Juliette Olivré, Bc, Sandrine Susini, PhD, Kevin Appourchaux, PhD, Christian Reimann, MD, PhD, Hanem Sadek, MSc, Olivier Pellé, MSc, Nicolas Cagnard, MSc, Elisa Magrin, MD, Chantal Lagresle-Peyrou, PhD, Tom Taghon, PhD, Antonio Rausell, PhD, Marina Cavazzana, MD, PhD, Isabelle André-Schmutz, PhD  Journal of Allergy and Clinical Immunology  Volume 141, Issue 4, Pages e4 (April 2018) DOI: /j.jaci Copyright © 2017 The Authors Terms and Conditions

2 Fig 1 Generation of T-cell progenitors from mPB CD34+ HSPCs. A-C, Phenotype, frequency, and cell numbers of day 3 and day 7 DL-4 cultures. D, Quantification of T-cell potential by T-cell differentiation on OP9/DL-1 cells in limiting dilution conditions. E-I, Gene expression profile of day 0 CD34+ cells and day 3 and day 7 CD7+ cells by using quantitative RT-PCR (Fig 1, E-G) or day 3 and day 7 CD7− and CD7+ cells (Fig 1, H and I). *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

3 Fig 1 Generation of T-cell progenitors from mPB CD34+ HSPCs. A-C, Phenotype, frequency, and cell numbers of day 3 and day 7 DL-4 cultures. D, Quantification of T-cell potential by T-cell differentiation on OP9/DL-1 cells in limiting dilution conditions. E-I, Gene expression profile of day 0 CD34+ cells and day 3 and day 7 CD7+ cells by using quantitative RT-PCR (Fig 1, E-G) or day 3 and day 7 CD7− and CD7+ cells (Fig 1, H and I). *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

4 Fig 2 In vivo reconstitution potential of T-cell precursors derived from mPB. A, Human thymic engraftment evaluated by the frequency of human CD45+ (hCD45) and mouse CD45+ (mCD45) lymphocytes in NSG recipients 6 weeks after transplantation with CD34+ cells before (day 0) or after 3 or 7 days of culture in DL-4 conditions. B, Presence of mature CD4+CD8+ double-positive cells in thymi of recipients transplanted with day 7 DL-4–cultured progenitors. The picture shows a representative photograph of recipient thymi injected with day 7 DL-4 cultured cells and day 0 HSPCs 8 weeks after transplantation. C, Presence of CD3+TCRαβ+ T cells in thymi and spleens of recipients injected with day 7 DL-4 cultured progenitors (gated on hCD45+ cells 8 weeks after transplantation). D, TCRβ VJ diversity in splenocytes from NSG mice transplanted with day 7 DL-4–cultured cells (8 weeks after transplantation). Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

5 Fig E1 Phenotype of adult CD34+ HSPCs exposed to 3 or 7 days of DL-4–Fc fusion protein compared with Fc alone. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

6 Fig E2 Presence of human CD25+CD4+Foxp3+CD127− regulatory T cells in the spleens of NSG recipients 8 weeks after transplantation of adult day 7 DL-4 precursors. Cells were first gated on 7AAD− live human CD45+ cells (left panel) and then on CD4+CD25+ cells (right panel). Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

Download ppt "Generation of adult human T-cell progenitors for immunotherapeutic applications  Laura Simons, MD, PhD, Kuiying Ma, MSc, Corinne de Chappedelaine, PhD,"

Similar presentations

Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,

Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,
Innate lymphoid cells contribute to allergic airway disease exacerbation by obesity  Laetitia Everaere, PhD, Saliha Ait-Yahia, PhD, Olivier Molendi-Coste,

Innate lymphoid cells contribute to allergic airway disease exacerbation by obesity  Laetitia Everaere, PhD, Saliha Ait-Yahia, PhD, Olivier Molendi-Coste,
Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 
Maternal phthalate exposure promotes allergic airway inflammation over 2 generations through epigenetic modifications  Susanne Jahreis, PhD, Saskia Trump,

Maternal phthalate exposure promotes allergic airway inflammation over 2 generations through epigenetic modifications  Susanne Jahreis, PhD, Saskia Trump,
Thymic stromal lymphopoietin signaling in CD4+ T cells is required for TH2 memory  Qun Wang, PhD, Jianguang Du, PhD, Jingjing Zhu, MSc, Xiaowei Yang, MSc,

Thymic stromal lymphopoietin signaling in CD4+ T cells is required for TH2 memory  Qun Wang, PhD, Jianguang Du, PhD, Jingjing Zhu, MSc, Xiaowei Yang, MSc,
B-cell reconstitution after lentiviral vector–mediated gene therapy in patients with Wiskott-Aldrich syndrome  Maria Carmina Castiello, PhD, Samantha.

B-cell reconstitution after lentiviral vector–mediated gene therapy in patients with Wiskott-Aldrich syndrome  Maria Carmina Castiello, PhD, Samantha.
IL-25 and CD4+ TH2 cells enhance type 2 innate lymphoid cell–derived IL-13 production, which promotes IgE-mediated experimental food allergy  Jee-Boong.

IL-25 and CD4+ TH2 cells enhance type 2 innate lymphoid cell–derived IL-13 production, which promotes IgE-mediated experimental food allergy  Jee-Boong.
An in vivo genetic reversion highlights the crucial role of Myb-Like, SWIRM, and MPN domains 1 (MYSM1) in human hematopoiesis and lymphocyte differentiation 

An in vivo genetic reversion highlights the crucial role of Myb-Like, SWIRM, and MPN domains 1 (MYSM1) in human hematopoiesis and lymphocyte differentiation 
Ting-ting Zhang, MSc, Klaus Okkenhaug, PhD, Baher F

Ting-ting Zhang, MSc, Klaus Okkenhaug, PhD, Baher F
Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma  Sonja Koch, PhD, Anna Graser, PhD, Hooman Mirzakhani,

Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma  Sonja Koch, PhD, Anna Graser, PhD, Hooman Mirzakhani,
Hypomorphic Janus kinase 3 mutations result in a spectrum of immune defects, including partial maternal T-cell engraftment  Federica Cattaneo, MD, Mike.

Hypomorphic Janus kinase 3 mutations result in a spectrum of immune defects, including partial maternal T-cell engraftment  Federica Cattaneo, MD, Mike.
Alefacept (lymphocyte function-associated molecule 3/IgG fusion protein) treatment for atopic eczema  Dagmar Simon, MD, Jennifer Wittwer, MD, Ganna Kostylina,

Alefacept (lymphocyte function-associated molecule 3/IgG fusion protein) treatment for atopic eczema  Dagmar Simon, MD, Jennifer Wittwer, MD, Ganna Kostylina,
Nazanin Farhadi, MSc, Laura Lambert, BA, Chiara Triulzi, PhD, Peter J

Nazanin Farhadi, MSc, Laura Lambert, BA, Chiara Triulzi, PhD, Peter J
Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,

Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,
Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 
IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,
B cells differentiate in human thymus and express AIRE

B cells differentiate in human thymus and express AIRE
Long-term immune reconstitution after matched unrelated hematopoietic stem cell transplantation for immunodeficiency  Vy Hong-Diep Kim, MD, Brenda Reid,

Long-term immune reconstitution after matched unrelated hematopoietic stem cell transplantation for immunodeficiency  Vy Hong-Diep Kim, MD, Brenda Reid,
Volume 13, Issue 2, Pages (August 2013)

Volume 13, Issue 2, Pages (August 2013)
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?

Similar presentations

Ads by Google