1. FIGURE 1A Abbott Architect i2000 (I) Abbott Architect i2000 (I) Ratio
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Ratio
10.0
9.0
8.0
7.0
6.0
Ratio
5.0
4.0
3.0
2.0
1.0
0.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

2. Abbott Architect i2000 (II) Abbott Architect i2000 (II)
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Ratio
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Ratio
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

3. Beckman Coulter Access Beckman Coulter Access
10.0
10.0
9.0
9.0
8.0
8.0
7.0
7.0
6.0
6.0
Ratio
Ratio
5.0
5.0
4.0
4.0
3.0
3.0
2.0
2.0
1.0
1.0
0.0
0.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

4. Siemens Coat a Count DPC Siemens Coat a Count DPC
10.0
10.0
9.0
9.0
8.0
8.0
7.0
7.0
6.0
6.0
Ratio
5.0
Ratio
5.0
4.0
4.0
3.0
3.0
2.0
2.0
1.0
1.0
0.0
0.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

5. Siemens ADVIA Centaur Siemens ADVIA Centaur Ratio Ratio
10.0
10.0
9.0
9.0
8.0
8.0
7.0
7.0
6.0
6.0
Ratio
5.0
Ratio
5.0
4.0
4.0
3.0
3.0
2.0
2.0
1.0
1.0
0.0
0.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

6. Siemens Immulite 2000 Siemens Immulite 2000 Ratio Ratio
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Ratio
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Ratio
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

7. Roche Cobas (II) Roche Cobas (II) Ratio Ratio ID-LC-MS/MS (nmol/L)
10.0
10.0
9.0
9.0
8.0
8.0
7.0
7.0
6.0
6.0
Ratio
Ratio
5.0
5.0
4.0
4.0
3.0
3.0
2.0
2.0
1.0
1.0
0.0
0.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

8. FIGURE 1B Abbott Architect i2000 (I) Abbott Architect i2000 (I) Ratio
2.0
2.0
1.8
1.8
1.6
1.6
1.4
1.4
1.2
1.2
Ratio
Ratio
1.0
1.0
0.8
0.8
0.6
0.6
0.4
0.4
0.2
0.2
0.0
0.0
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

9. Abbott Architect i2000 (II) Abbott Architect i2000 (II)
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
Ratio
Ratio
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

10. Beckman Coulter Access Beckman Coulter Access
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
2.0
1.8
1.6
1.4
1.2
Ratio
Ratio
1.0
0.8
0.6
0.4
0.2
0.0
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

11. Siemens Coat a Count DPC Siemens Coat a Count DPC
2.0
2.0
1.8
1.8
1.6
1.6
1.4
1.4
1.2
1.2
Ratio
1.0
Ratio
1.0
0.8
0.8
0.6
0.6
0.4
0.4
0.2
0.2
0.0
0.0
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

12. Siemens ADVIA Centaur Siemens ADVIA Centaur Ratio Ratio
2.0
2.0
1.8
1.8
1.6
1.6
1.4
1.4
1.2
1.2
Ratio
Ratio
1.0
1.0
0.8
0.8
0.6
0.6
0.4
0.4
0.2
0.2
0.0
0.0
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)

13. Siemens Immulite 2000 Siemens Immulite 2000 Ratio Ratio
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
Ratio
Ratio
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
ID-LC-MS/MS (nmol/L)
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
ID-LC-MS/MS (nmol/L)

14. Roche Cobas (II) Roche Cobas (II) Ratio Ratio ID-LC-MS/MS (nmol/L)
2.0
2.0
1.8
1.8
1.6
1.6
1.4
1.4
1.2
1.2
Ratio
Ratio
1.0
1.0
0.8
0.8
0.6
0.6
0.4
0.4
0.2
0.2
0.0
0.0
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
4.0
8.0
12.0
16.0
20.0
24.0
28.0
32.0
36.0
40.0
ID-LC-MS/MS (nmol/L)
ID-LC-MS/MS (nmol/L)