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Inhibition of MicroRNA-204 Conducts Neuroprotection Against Spinal Cord Ischemia  Lihui Yan, MD, Enyi Shi, MD, PhD, Xiaojing Jiang, MD, PhD, Jiang Shi,

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Presentation on theme: "Inhibition of MicroRNA-204 Conducts Neuroprotection Against Spinal Cord Ischemia  Lihui Yan, MD, Enyi Shi, MD, PhD, Xiaojing Jiang, MD, PhD, Jiang Shi,"— Presentation transcript:

1 Inhibition of MicroRNA-204 Conducts Neuroprotection Against Spinal Cord Ischemia 
Lihui Yan, MD, Enyi Shi, MD, PhD, Xiaojing Jiang, MD, PhD, Jiang Shi, MD, Shilun Gao, MD, Hongbo Liu, MD, PhD  The Annals of Thoracic Surgery  Volume 107, Issue 1, Pages (January 2019) DOI: /j.athoracsur Copyright © 2019 The Society of Thoracic Surgeons Terms and Conditions

2 Fig 1 Experimental groups and protocol. (A) Measurement of expressions of miR-204, LC3-II/LC3-I, beclin-1, and BCL-2 at different time points after spinal cord ischemia. (B) Experimental protocol for evaluation of the neurologic protection induced by inhibition of miR-204. (3-MA = 3-Methyladenine; TU = transfection unit; TUNEL = terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling.) The Annals of Thoracic Surgery  , 76-83DOI: ( /j.athoracsur ) Copyright © 2019 The Society of Thoracic Surgeons Terms and Conditions

3 Fig 2 Time course of expressions of miR-204, beclin-1, BCL-2, and the LC3-II/LC3-I ratio in spinal cords after reperfusion. (A) miR-204 expression. (B) Western blot pictures showing the expressions of LC3-I and LC3-II and densitometric quantification of the LC3-II/LC3-I ratio. (C) Western blot pictures and densitometric quantification of the expression beclin-1. (D) Western blot pictures and densitometric quantification of the expression of BCL-2. *p < 0.05 versus the sham group. The Annals of Thoracic Surgery  , 76-83DOI: ( /j.athoracsur ) Copyright © 2019 The Society of Thoracic Surgeons Terms and Conditions

4 Fig 3 Hind-limb motor function assessed with motor deficit index. *p < versus the control group at the same observation point. #p < versus the antagomiR-204 group at the same observation point. (3-MA = 3-Methyladenine.) The Annals of Thoracic Surgery  , 76-83DOI: ( /j.athoracsur ) Copyright © 2019 The Society of Thoracic Surgeons Terms and Conditions

5 Fig 4 Expressions of miR-204, beclin-1, BCL-2, caspase-3, and the LC3-II/LC3-I ratio in spinal cords after transient ischemia. (A) miR-204 expression. (B) Representative Western blot pictures showing the expressions of LC3-I and LC3-II and densitometric quantification of the LC3-II/LC3-I ratio. (C) Western blot pictures and densitometric quantification of beclin-1 expression. (D) Western blot pictures and densitometric quantification of BCL-2 expression. (E) Western blot pictures and densitometric quantification of caspase-3 expression. *p < 0.05 versus the control group. †p < 0.05 versus the sham group. #p < 0.05 versus the antagomiR-204 group. (3-MA = 3-Methyladenine.) The Annals of Thoracic Surgery  , 76-83DOI: ( /j.athoracsur ) Copyright © 2019 The Society of Thoracic Surgeons Terms and Conditions

6 Fig 5 Histologic examinations of lumber spinal cords. (A) Representative hematoxylin and eosin and TUNEL staining sections. a and d, Control group; b and e, antagomiR-204 group; c and f, antagomiR MA group. Black triangle shows TUNEL-positive neurons. (B) Number of viable motor neurons in the ventral gray matter of lumber spinal cords. (C) Number of apoptotic cells in the ventral gray matter of lumber spinal cords. *p < versus the control group. †p < versus the sham group. #p < versus the antagomiR-204 group. (3-MA = 3-Methyladenine; TUNEL = terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling.) The Annals of Thoracic Surgery  , 76-83DOI: ( /j.athoracsur ) Copyright © 2019 The Society of Thoracic Surgeons Terms and Conditions

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