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Osteoarthritis and Cartilage

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1 Osteoarthritis and Cartilage
Isolation of phenotypically and functionally distinct endogeneous human bone marrow- derived mesenchymal stem/stromal cell subsets  K. Sivasubramaniyan, A. Harichandan, P. Boss, H.-J. Buehring, G. van Osch  Osteoarthritis and Cartilage  Volume 24, (April 2016) DOI: /j.joca Copyright © Terms and Conditions

2 Figure, A) staining of BM cells with CD45, CD271, and CD56, gating on the CD45- subset and analyzed for expression of CD271 and CD56. Sort windows were set as shown in the plot A. B) After sorting, cells were cultured and differentiated into osteoblasts (Alkaline phosphatase (ALP), adipocytes (Oil Red O) and chondrocytes (Alcian Blue). C) CD271 (marked by arrowhead) and CD56 (marked by arrow) are co-expressed on bone-lining MSCs. Perivascular MSCs express CD271 and are negative for CD56. Scale bars: 20 μm. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © Terms and Conditions


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