1. Clinical Microbiology and Infection
How to: EUCAST recommendations on the screening procedure E.Def 10.1 for the detection of azole resistance in Aspergillus fumigatus isolates using four-well azole- containing agar plates 
J. Guinea, P.E. Verweij, J. Meletiadis, J.W. Mouton, F. Barchiesi, M.C. Arendrup M.C. Arendrup, S. Arikan-Akdagli, F. Barchiesi, M. Castanheira, E. Chryssanthou, N. Friberg, J. Guinea, H. Järv, N. Klimko, O. Kurzai, K. Lagrou, C. Lass-Flörl, M. Mares, T. Matos, J. Meletiadis, C.B. Moore, J.W. Mouton, K. Muehlethaler, T.R. Rogers, C.T. Andersen, A. Velegraki J. Guinea, P.E. Verweij, J. Meletiadis, J.W. Mouton, F. Barchiesi, M.C. Arendrup M.C. Arendrup, S. Arikan-Akdagli, F. Barchiesi, M. Castanheira, E. Chryssanthou, N. Friberg, J. Guinea, H. Järv, N. Klimko, O. Kurzai, K. Lagrou, C. Lass-Flörl, M. Mares, T. Matos, J. Meletiadis, C.B. Moore, J.W. Mouton, K. Muehlethaler, T.R. Rogers, C.T. Andersen, A. Velegraki 
Clinical Microbiology and Infection 
DOI: /j.cmi
Copyright © 2018 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

2. Fig. 1
Four-well agar plate with wells filled out with 1 mL of agar media-containing azoles (wells 1–3) or antifungal-free agar (well 4) used as growth control. ITC, itraconazole; VRC, voriconazole; POS, posaconazole.
Clinical Microbiology and Infection DOI: ( /j.cmi )
Copyright © 2018 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

3. Fig. 2
Expected growth pattern for the A. fumigatus SSI-4524 QC strain harbouring the TR34/L98H alteration in the CYP51A gene and promoter. Each well of the plate contains the following azoles: well 1 (itraconazole 4 mg/L), well 2 (voriconazole 2 mg/L), well 3 (posaconazole 0.5 mg/L), and well 4 (no azoles, growth control). SSI = Statens Serum Institut, Copenhagen, Denmark. The QC strain is available from the EUCAST Development Laboratory (EDL) for fungi:
Clinical Microbiology and Infection DOI: ( /j.cmi )
Copyright © 2018 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

4. Fig. 3
Expected growth pattern for the A. fumigatus SSI-5586 QC strain harbouring a G54W substitution in the CYP51A gene. Each well of the plate contains the following azoles: well 1 (itraconazole 4 mg/L), well 2 (voriconazole 2 mg/L), well 3 (posaconazole 0.5 mg/L), and well 4 (no azoles, growth control). SSI, Statens Serum Institut, Copenhagen, Denmark. The QC strain is available from the EUCAST Development Laboratory (EDL) for fungi:
Clinical Microbiology and Infection DOI: ( /j.cmi )
Copyright © 2018 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions