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Nipple-areolar pigmentation: histology and potential for reconstitution in breast reconstruction  Nicola Dean, Julie Haynes, Jim Brennan, Tim Neild, Chris.

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Presentation on theme: "Nipple-areolar pigmentation: histology and potential for reconstitution in breast reconstruction  Nicola Dean, Julie Haynes, Jim Brennan, Tim Neild, Chris."— Presentation transcript:

1 Nipple-areolar pigmentation: histology and potential for reconstitution in breast reconstruction 
Nicola Dean, Julie Haynes, Jim Brennan, Tim Neild, Chris Goddard, Bronwyn Dearman, Rod Cooter  British Journal of Plastic Surgery  Volume 58, Issue 2, Pages (March 2005) DOI: /j.bjps Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

2 Figure 1 Nipple-areolar reconstruction with a pigmented graft, (A) a few weeks after surgery, and (B) 2 years after surgery. British Journal of Plastic Surgery  , DOI: ( /j.bjps ) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

3 Figure 2 Schematic representation of protocol for tissue sections.
British Journal of Plastic Surgery  , DOI: ( /j.bjps ) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

4 Figure 3 Masson–Fontana staining of areolar skin to show melanin (×20). British Journal of Plastic Surgery  , DOI: ( /j.bjps ) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

5 Figure 4 Image analysis of Masson–Fontana stained sections (areolar skin) (×20). British Journal of Plastic Surgery  , DOI: ( /j.bjps ) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

6 Figure 5 Staining of breast skin with Mel-5 antibody, as seen with the image analysis software (melanocytes arrowed) (×20). British Journal of Plastic Surgery  , DOI: ( /j.bjps ) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

7 Figure 6 Growth of melanocytes in medium containing cholera toxin and PMA vs no cholera toxin or PMA. British Journal of Plastic Surgery  , DOI: ( /j.bjps ) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions


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