1. Volume 7, Issue 1, Pages 256-259 (January 2014)
A Passive Repression Mechanism that Hinders Synergic Transcriptional Activation by Heat Shock Factors Involved in Sunflower Seed Longevity 
Javier Tejedor-Cano, Raúl Carranco, José-María Personat, Pilar Prieto-Dapena, Concepción Almoguera, José Manuel Espinosa, Juan Jordano 
Molecular Plant 
Volume 7, Issue 1, Pages (January 2014)
DOI: /mp/sst117
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
Passive Repression by HaIAA27 of the Synergism between HaHSFA9 and HaHSFA4a.
(A, B) Mostly cytosolic localization of HaHSFA4a:GFP (A) and enhanced nuclear localization of the NES mutant protein HaHSFA4a(NESmut):GFP (B).
(C–E) Co-expression of HaHSFA4a:GFP with HaHSFA9 (C), or with the hybrid HSF A2:A92 (D), but not with the hybrid HSF A2:A91 (E) enhanced nuclear localization of HaHSFA4a:GFP. Assays performed in leaves of Nicotiana benthamiana. Bars = 100 μm.
(F) A synergistic transcriptional activation with HaHSFA4a (A4a) was observed using HaHSFA9 (A4a+A9), or with A2:A92 (A4a+A2:A92), but not with the rest of HSFs analyzed in bombarded sunflower leaves, individually or in combination: none (–), LpHSFA2 (A2), hybrid HSF A2:A91 (A2:A91).
(G–I) BiFC interaction in bombarded sunflower embryos; HaIAA27:YFPN + HaHSFA4a:YFPC (G); HaHSFA4a:YFPN + HaHSFA9:YFPC (H); HaHSFA4a:YFPN + HaHSFA9:YFPC + HaIAA27mIIab (I). Bars = 30 μm.
(J) The WT and mutant domain I (mI) HaIAA27 (I27) proteins fully abolished the synergism between HaHSFA4a (A4a) and HaHSFA9 (A9). Analyses performed in bombarded sunflower embryos with the indicated combination of transcription factors.
(K) Active repression assays: a, WT, HaIAA27:GAL4-DB fusion protein (I27), but not the rest of analyzed HaIAA27 fusion proteins (maps on top) efficiently repressed 35S-induced transcription.
Maps of the G4 reporter gene and of the different effectors used in (F) and (H) are shown at the top of the figure. Numbers in parentheses show the number of replicates for each reporter/effector combination. The same bar shading indicates similar reporter activity. Error bars denote the SE. Asterisks indicate statistically significant synergism.
Molecular Plant 2014 7, DOI: ( /mp/sst117)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions