1. Volume 57, Issue 6, Pages 2626-2633 (June 2000)
Pentoxifylline inhibits human peritoneal mesothelial cell growth and collagen synthesis: Effects on TGF-β 
Cheng-Chung Fang, Chung-Jen Yen, Yung-Ming Chen, Ren-Shi Shyu, Tun-Jun Tsai, Po-Huang Lee, Bor-Shen Hsieh 
Kidney International 
Volume 57, Issue 6, Pages (June 2000)
DOI: /j x
Copyright © 2000 International Society of Nephrology Terms and Conditions

2. Figure 1
Pentoxifylline (PTX) inhibits human peritoneal mesothelial cell (HPMC) growth. (A) Time response curve. Various concentrations of PTX [0 (▪), 0.01 (•), 0.03 (♦), 0.1 (▾), and 0.3 (▴) mg/mL] were added after overnight plating of HPMCs and the cells were further incubated for 1 to 5 days, and then an MTT assay was performed. Data represent one of the 3 experiments. (B) Dose response curve. Cells were incubated for 5 days after addition of various concentrations of PTX, and then an MTT assay was performed. All data are expressed as the means with SD of 3 experiments conducted in pentaplicate. *P < 0.05 vs. control.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

3. Figure 2
Collagen synthesis of HPMCs in the presence of various concentrations of PTX. The result is expressed as the percentage of the control. All data are expressed as the means with SD of 3 experiments conducted in triplicate. Details of the method are in the text. *P < 0.05 vs. control.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

4. Figure 3
Northern blot analysis of mRNA isolated from HPMC incubated with or without PTX. The RNA blots were hybridized with procollagen α1 (I) and GAPDH cRNA probes. Cells were treated with PTX 0.3 mg/mL for 4 hours (1), 0.3 mg/mL for 8 hours (2), 0.3 mg/mL for 16 hours (3), 0.1 mg/mL for 16 hours (4), 0.03 mg/mL for 16 hours (5), and 0.3 mg/mL for 24 hours (6). C is control.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

5. Figure 4
Northern blot analysis of mRNA isolated from HPMC. The RNA blots were hybridized with procollagen α1 (I) and GAPDH cRNA probes (A), or procollagen α1 (III) and GAPDH cRNA probes (B). Cells were treated with agents for 16 hours. Control (1), 1 ng/mL of TGF-β (2), 1 ng/mL of TGF-β and 0.3 mg/mL of PTX (3).
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

6. Figure 5
MTT assay on the fifth day after adding 10 μmol/L of H-89 (pretreated for 30 min) with and without 0.1 mg/mL of PTX. All data are expressed as the means with SD of 3 experiments conducted in pentaplicate. Abbreviations are: I, initial plating at day 0; C, control; PTX, 0.1 mg/mL of PTX; H, 10 μmol/L of H-89. *P < 0.05 vs. control.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

7. Figure 6
Northern blot analysis of mRNA isolated from HPMC. The RNA blots were hybridized with procollagen α1 (I) and GAPDH cRNA probes and GAPDH cRNA probes. Cells were pretreated with 10 μmol/L of H-89 and then with/without 0.1 mg/mL of PTX for 16 hours. Control (C), 0.1 mg/mL of PTX (1), 10 μmol/L of H-89 (2), and 10 μmol/L of H-89 with 0.1 mg/mL of PTX (3).
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

8. Figure 7
MTT assay on the fifth day after adding 10 μmol/L of indomethacin with and without 0.1 mg/mL of PTX. All data are expressed as the means with SD of 3 experiments conducted in pentaplicate. Abbreviations are: I, initial plating at day 0; C, control; PTX, 0.1 mg/mL of PTX; Id, 10 μmol/L of indomethacin. *P < 0.05 vs. control.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions