1. Volume 9, Issue 4, Pages 467-473 (October 1998)
CD8 Expression Allows T Cell Signaling by Monomeric Peptide-MHC Complexes 
Jérôme Delon, Claude Grégoire, Bernard Malissen, Sylvie Darche, Fabrice Lemaître, Philippe Kourilsky, Jean-Pierre Abastado, Alain Trautmann 
Immunity 
Volume 9, Issue 4, Pages (October 1998)
DOI: /S (00)

2. Figure 1 FPLC Profile of the Kd-HA Preparation
The arrows indicate the positions for monomers and dimers. The molecular weight markers were thyroglobulin (670 kDa), bovine gamma globulin (158 kDa), chicken ovalbumin (44 kDa) and equine myoglobin (17 kDa).
Immunity 1998 9, DOI: ( /S (00) )

3. Figure 2 Ca2+ Responses in Transgenic T Lymphocytes
(A) Ca2+ responses elicited by different concentrations (0.3 to 10 μg/ml) of monomers of Kd-HA in primed CD8+ T cells purified from CL4 transgenic mice. The trace show the averages of all responding cells. The arrow indicates the time when Kd-HA is added.
(B) Fab fragments of the anti-Kd MAb SF prevent the CD8-Kd interaction and inhibit the Ca2+ response elicited by 10 μg/ml Kd-HA.
(C) Percentage of CL4 T cells presenting a [Ca2+]i increase in response to 10 μg/ml anti-CD3 MAb, or to 10 μg/ml monomeric Kd-HA, in the absence or presence of SF With 10 μg/ml, both anti-CD3 and Kd-HA stimulations were submaximal, as judged by the percentage of responding cells. Data are from 3 independent experiments.
Immunity 1998 9, DOI: ( /S (00) )

4. Figure 3 Phenotype of Hybridoma T Cells
FACS analysis of the expression of CD3 (KT3), CD8α (H ) and CD8β (H ) in T1.4, T1.4 CD8αβ and T1.4 CD8α′β transfectants.
Immunity 1998 9, DOI: ( /S (00) )

5. Figure 4 Ca2+ Responses in Hybridoma T Cells
Ca2+ responses elicited by monomers of Kd-ABA in CD8αβ+ T cells (A), in CD8− T cells (B) and in CD8α′β+ T cells (D). The response of the three transfectants to 10 μg/ml anti-CD3 stimulation is shown in C. Data are from 5 independent experiments.
Immunity 1998 9, DOI: ( /S (00) )

6. Figure 5 Ca2+ Responses to Dimers of Kd-ABA
Comparison of Ca2+ responses evoked in CD8αβ+, CD8α′β+, and CD8α−β− T cells. (A): Responses to dimers of Kd-ABA (10 μg/ml). (B): Percentage of responding T cells following stimulation by anti-CD3, monomers or dimers of Kd-ABA. Data are from 3 independent experiments.
Immunity 1998 9, DOI: ( /S (00) )