1. Prolonged Ischemia Is Associated With More Pronounced Rejection in the Lung Allograft 
Cyril Serrick, MSc, Adel Giaid, PhD, Alex Reis, MD, Hani Shennib, MD 
The Annals of Thoracic Surgery 
Volume 63, Issue 1, Pages (January 1997)
DOI: /S (96)

2. Fig. 1
(a–c) Photomicrographs showing major histocompatibility complex (MHC) class II immunostaining. (a) Lung biopsy specimen from 4-hour ischemia group 1 week postoperatively with peribronchial tissue staining (arrow). (b) Biopsy specimen from 24-hour ischemia group 24 hours after reperfusion showing diffuse expression of MHC class II reactivity in airway epithelium. (c) Interstitial inflammatory cells in lung biopsy specimen 1 week after transplantation in 24-hour ischemia group. (d–f) Hematoxylin and eosin staining of lung biopsy specimens. (d). Grade 1-2 rejection with a perivascular mononuclear cell infiltrate in a lung allograft biopsy specimen from 4-hour ischemia group 1 week after transplantation. (e) Lung tissue biopsy specimen showing diffuse aveolar edema, hemorrhage, and inflammatory cells in interstitum from 24-hour ischemia group 24 hours after transplantation. (f) Lung biopsy specimen showing perivascular infiltration representing grade 3 rejection 1 week after transplantation in 24-hour ischemia group. (a–d, f ×400; e ×200; all before 9% reduction.)
The Annals of Thoracic Surgery  , DOI: ( /S (96) )

3. Fig. 2
Major histocompatibility complex class II staining of bronchial epithelium. Data are shown as the mean ± the standard error of the mean. (∗ = p < 0.05 compared with preoperative value; †† = p < 0.05 compared with 4-hour ischemia group; † = p < 0.01 compared with 24; ∗∗ = p < compared with preoperative, 1 hour and 4 hours.)
The Annals of Thoracic Surgery  , DOI: ( /S (96) )

4. Fig. 3
Major histocompatibility complex class II staining of cellular infiltrate. Data are shown as the mean ± the standard error of the mean. (∗ = p < 0.05 compared with preoperative and 1 hour values; † = p < 0.01 compared with 4-hour ischemia group.)
The Annals of Thoracic Surgery  , DOI: ( /S (96) )

5. Fig. 4
Major histocompatibility complex class II staining of vascular endothelium. Data are shown as the mean ± the standard error of the mean. († = p < 0.01 compared with 4-hour ischemia group; ∗ = p < 0.05 compared with preoperative and 1 hour values; †† = p < compared with 4-hour ischemia group.)
The Annals of Thoracic Surgery  , DOI: ( /S (96) )

6. Fig. 5
Cytokine levels in bronchoalveolar lavage fluid from 4-hour ischemia group lung allografts. Values are shown as a percentage of preoperative values (mean ± standard error of the mean). (IL-2 = interleukin-2; IFNγ = interferon-gamma; ∗ = p < 0.05 compared with preoperative value.)
The Annals of Thoracic Surgery  , DOI: ( /S (96) )

7. Fig. 6
Cytokine levels in bronchoalveolar lavage fluid from 24-hour ischemia group lung allografts. Values are shown as a percentage of preoperative values (mean ± standard error of the mean). (IL-2 = interleukin-2; IFNγ = interferon-gamma; ∗ = p < 0.05 compared with preoperative value.)
The Annals of Thoracic Surgery  , DOI: ( /S (96) )