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Polymerase Chain Reaction

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Presentation on theme: "Polymerase Chain Reaction"— Presentation transcript:

1 Polymerase Chain Reaction

2 DNA

3 PCR DNA replication can also be performed in vitro (artificially, outside a cell). The polymerase chain reaction (PCR), a common laboratory technique, cyclically applies such artificial synthesis to amplify a specific target DNA fragment from a pool of DNA. Kary Mullis 1993

4 PCR

5 PCR

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8 Temperate procedure

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14 Thermocyclers Conventional PCR

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17 Detection of genetic mutations
Virus detection and Genotyping of the hepatitis virus Help in the diagnosis of certain diseases . Used in cloning and Sequencer of DNA Detection the length of DNA Technology of c DNA (complementary DNA ( Determine the desired gene from a mixture of genes Human genome project Diagnosed of cancer Organ transplantation


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