1. Longitudinal Analysis of T-Cell Receptor Variable β Chain Repertoire in Patients with Acute Graft-versus-Host Disease after Allogeneic Stem Cell Transplantation 
Congxiao Liu, Min He, Barbara Rooney, Thomas B. Kepler, Nelson J. Chao 
Biology of Blood and Marrow Transplantation 
Volume 12, Issue 3, Pages (March 2006)
DOI: /j.bbmt
Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

2. Figure 1
CDR3 size distribution patterns in PBMCs from individual healthy humans. Total RNA from PBMCs was reverse-transcribed and amplified by PCR with 23 pairs of TCRBV- and Cβ-specific primers. The PCR product was further copied in runoff reactions by a fluorescent Cβ primer and loaded to electrophoresis on an automated DNA sequencer. The size and intensity of each peak were determined with the help of the Immunoscope software package (PE Applied Biosystems).
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

3. Figure 2
A representative composite spectratyping of the TCRBV gene at serial time points after allo-PBSCT. TCRBV subfamilies are shown on the x-axis, and peak numbers per family are shown on the y-axis. A, TCRBV repertoire in a patient with acute GVHD. The peak numbers in most of the TCRBV subfamilies were less than 4. B, TCRBV repertoire in a patient without acute GVHD. The peak numbers in most of the TCRBV subfamilies were more than 5.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

4. Figure 2
A representative composite spectratyping of the TCRBV gene at serial time points after allo-PBSCT. TCRBV subfamilies are shown on the x-axis, and peak numbers per family are shown on the y-axis. A, TCRBV repertoire in a patient with acute GVHD. The peak numbers in most of the TCRBV subfamilies were less than 4. B, TCRBV repertoire in a patient without acute GVHD. The peak numbers in most of the TCRBV subfamilies were more than 5.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

5. Figure 3
Comparison of the extent of perturbation in the TCRBV repertoire between groups with and without GVHD from 6 weeks to 6 months after transplantation. A, Divergence attributable to differences in GVHD status as a function of time since transplantation, estimated as described in the text (triangles, GVHD; circles, no GVHD). Corrected refers to the subtraction of components attributable to predictors other than GVHD and time. Increasing divergence is equivalent to greater perturbation from the healthy profile and decreased diversity. Note that the 2 populations are indistinguishable before transplantation and again at 6 months after transplantation. Error bars represent 95% confidence intervals. B, The 95% confidence intervals for the corrected mean difference between GVHD and non-GVHD patient divergences, as described in the text. Corrected again refers to the subtraction of components of predictors other than TCRBV and GVHD. Those confidence intervals that do not include the origin correspond to hypothesis tests in which the null hypothesis, that the true mean difference is 0, was rejected.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

6. Figure 4
Spectratyping profile of TCRBV7, 11, 15, 16, 17, and 23 in recipients with or without acute GVHD at serial time points after allo-PBSCT. Results are shown for each TCRBV family as a density peak histogram. CDR3 sizes are shown on the x-axis, and the peak fluorescence intensity is shown on the y-axis.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions