1. ZIKV infection induces global DNA methylation changes in multicellular human cerebral organoids.
ZIKV infection induces global DNA methylation changes in multicellular human cerebral organoids. (A) (Top) Schematic of the samples used for whole-genome bisulfite sequencing (WGBS). (Bottom) ZIKV-positive cells (anti-flavivirus group E antigen, green) upon ZIKV infection (strain MR766) of cerebral organoid-derived 2D cultures. Bars, 100 µm. (B) Heat maps show methylation levels of all defined 1-kb tiles (left) and DMRs with an average methylation difference greater than 0.2 (right). (C) Enrichment [log2(hypergeometric P value)] of genomic features of differentially methylated 1-kb tiles (q value of <0.05 and methylation difference greater than 0.2). UTR, untranslated region; ncRNA, noncoding RNA; TTS, transcription termination site; SINE, short interspersed nuclear element; LINE, long interspersed nuclear element; LTR, long terminal repeat. (D) Examples of a hypomethylated (DDX3X) and hypermethylated (IRX3) gene locus. Gray, areas with nonsignificant sequencing signal; red dashed line, differentially methylated region.
Sylvie Janssens et al. mSystems 2018; doi: /mSystems