1. MSI and cutin and suberin composition analyses in reticulated and in-between smooth regions of fruit skin tissues.
MSI and cutin and suberin composition analyses in reticulated and in-between smooth regions of fruit skin tissues. A, Light microscopy analysis of fruit skin samples analyzed using MALDI-MSI. B, MALDI-MSI showing the spatial distribution of ferulic acid ( ± mass-to-charge ratio [m/z]) located in the reticulated areas of reticulated skin samples but absent in the smooth skin samples. Dashed white lines represent skin surface areas analyzed by MALDI-MSI. Bars in A and B = 1 mm. C, Manual dissection of reticulated regions and the smooth regions in-between reticulated ones from cv Sharlyn fruit skin. D, Total cutin and suberin contents in skin tissues dissected as shown in C. E, Levels of individual cutin and suberin monomers in skin tissues dissected as in C. Cutin and suberin profiles in D and E were analyzed by GC-MS; y axes represent relative peak areas following normalization to a C32-alkane internal standard. Data represent means ± se of three biological replicates each per sample (generated from a pool of skin tissues from four different fruit). Significance was calculated according to Student’s t test: *, P < 0.05; **, P < 0.001; and ***, P <
Hagai Cohen et al. Plant Physiol. 2019;179:
©2019 by American Society of Plant Biologists