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Activity–rest stimulation of latissimus dorsi for cardiomyoplasty: 1-year results in sheep  Giorgio Arpesella, MD, Ugo Carraro, MD, Piero M Mikus, MD,

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Presentation on theme: "Activity–rest stimulation of latissimus dorsi for cardiomyoplasty: 1-year results in sheep  Giorgio Arpesella, MD, Ugo Carraro, MD, Piero M Mikus, MD,"— Presentation transcript:

1 Activity–rest stimulation of latissimus dorsi for cardiomyoplasty: 1-year results in sheep 
Giorgio Arpesella, MD, Ugo Carraro, MD, Piero M Mikus, MD, Franco Dozza, MD, Pierloca Lombardi, MD, Giuseppe Marinelli, Sandra Zampieri, Abdul H El Messlemani, Katia Rossini, Angelo Pierangeli, MD  The Annals of Thoracic Surgery  Volume 66, Issue 6, Pages (December 1998) DOI: /S (98)

2 Fig 1 Cardiomyoplasty-like mobilization of sheep latissimus dorsi and daily continuous or activity–rest stimulation. From its natural insertions to ribs and spinal column (interrupted line) the latissimus dorsi was resutured to ribs in the shortened position it spontaneously attained. The Annals of Thoracic Surgery  , DOI: ( /S (98) )

3 Fig 2 Cardiomyoplasty-like mobilization of sheep latissimus dorsi and daily continuous or activity–rest stimulation. Anatomic records. Normal latissimus dorsi from sheep: (B) 5/95; (D) 6/95; latissimus dorsi after 12 months of 10-hour/day-stimulation from sheep: (A) 5/95; (C) 6/95; (E) 4/95; (F) 1/95; latissimus dorsi after 6 months of 24-hour/day stimulation followed by a 6-month rest: (G) 2/95; (H) 3/95. (I) Cross section of the muscles. Although slightly diminished in size and weight, after 1 year of daily activity–rest stimulation the latissimus dorsi appear trophic and as red as the normal contralateral muscles. The Annals of Thoracic Surgery  , DOI: ( /S (98) )

4 Fig 3 Cardiomyoplasty-like mobilization of sheep latissimus dorsi and daily continuous or activity–rest stimulation. Hematoxylin and eosin stain (A, C, E) and pH 4.35 ATPase (B, D, F) of distal samples of latissimus dorsi. Six months of 24-hour/day stimulation: (A) sheep 2/95 (hematoxylin and eosin); (B) sheep 2/95 (ATPase); 6 months of 10-hour/day stimulation: (C) sheep 6/95 (hematoxylin and eosin); (D) sheep 6/95 (ATPase); (E) normal ATPase latissimus dorsi (hematoxylin and eosin); (F) normal latissimus dorsi (ATPase). (Hematoxylin and eosin; ×100 before 14% reduction, ATPase; ×80 before 14% reduction.) The Annals of Thoracic Surgery  , DOI: ( /S (98) )

5 Fig 4 Cardiomyoplasty-like mobilization of sheep latissimus dorsi and daily continuous or activity–rest stimulation. (Hematoxylin and eosin; ×100 before 8% reduction.) Distal samples of sheep latissimus dorsi after 6 months of 24-hour/day stimulation followed by 6 months of rest: (A) sheep 2/95; (B) sheep 3/95; latissimus dorsi after 12 months of 10-hour/day stimulation: (C) sheep 1/95; (D) sheep 4/95; (E) sheep 5/95; (F) sheep 6/95. The Annals of Thoracic Surgery  , DOI: ( /S (98) )

6 Fig 5 Cardiomyoplasty-like mobilization of sheep latissimus dorsi and daily continuous or activity–rest stimulation. (ATPase pH 10.4 ×80 before 14% reduction.) Distal samples of latissimus dorsi after 6 months of 24-hour/day stimulation followed by 6 months of rest: (A) sheep 2/95; (B) sheep 3/95; latissimus dorsi after 12 months of 10-hour/day stimulation: (C) sheep 1/95; (D) sheep 4/95; (E) sheep 5/95; (F) sheep 6/95. The Annals of Thoracic Surgery  , DOI: ( /S (98) )


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