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Mast Cells Are Required for Full Expression of Allergen/SEB-Induced Skin Inflammation  Tomoaki Ando, Kenji Matsumoto, Siavash Namiranian, Hirotaka Yamashita,

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Presentation on theme: "Mast Cells Are Required for Full Expression of Allergen/SEB-Induced Skin Inflammation  Tomoaki Ando, Kenji Matsumoto, Siavash Namiranian, Hirotaka Yamashita,"— Presentation transcript:

1 Mast Cells Are Required for Full Expression of Allergen/SEB-Induced Skin Inflammation 
Tomoaki Ando, Kenji Matsumoto, Siavash Namiranian, Hirotaka Yamashita, Haley Glatthorn, Miho Kimura, Brandon R. Dolan, James J. Lee, Stephen J. Galli, Yuko Kawakami, Colin Jamora, Toshiaki Kawakami  Journal of Investigative Dermatology  Volume 133, Issue 12, Pages (December 2013) DOI: /jid Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 T cells, but not B cells, are indispensable for maximal skin inflammation. Dermatitis induction by epicutaneous applications of Dermatophagoides farinae extract (Der f) and Staphylococcal enterotoxin B (SEB) was performed as described in the Materials and Methods. Each symbol represents one mouse. (a) Clinical skin scores. (b) Hematoxylin and eosin (H&E) staining of naive (upper rows) and lesional (lower rows) skin tissues. Bar=200μm. (c) Thicknesses of epidermis, dermis, and total skin (epidermis+dermis) layers, as measured in H&E-stained tissues. (d) Relationships between clinical scores and skin layer thicknesses. Linear regression lines are shown. *P<0.05, **P<0.01, ***P<0.001, and ****P< AD, atopic dermatitis; NS, not significant; WT, wild type. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Eosinophils are dispensable for allergen-induced skin inflammation. (a, b) Eosinophils stained with Congo red were enumerated in the skin sections derived from experiments shown in Figure 1. Each symbol represents one mouse. There was no significant correlation between eosinophil numbers and clinical scores. (c, d) Dermatitis induction by epicutaneous applications of Dermatophagoides farinae extract and Staphylococcal enterotoxin B (SEB) was performed on eosinophil-deficient (c) PHIL and (d) ΔdblGATA mice. Clinical scores are shown. AD, atopic dermatitis; HPF, high power field; NS, not significant; WT, wild type. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Mast cells are indispensable for maximal skin inflammation. (a) Mast cell–deficient KitW-sh/W-sh mice exhibited lower clinical scores than wild-type (WT) mice. The scores similar to WT mice were restored by engraftment of bone marrow–derived mast cells (BMMCs; W-sh+BMMC). (b) Hematoxylin and eosin (H&E) staining of naive and lesional skin tissues. Enlarged images of the areas indicated by rectangles are shown below. Bar=200μm. (c) Thicknesses of epidermis, dermis, and total skin layers. (d) Immunofluorescence microscopy was performed on naive and lesional skin tissues. Numbers of (e) eosinophils and (f) neutrophils before and after atopic dermatitis (AD) induction. *P<0.05, **P<0.01, and ***P< Col, collagen; Ecad, E-cadherin; HPF, high power field; K, keratin; Lor, loricrin. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 FcεRI, but not tumor necrosis factor-α (TNF-α), is required for maximal skin inflammation. Dermatophagoides farinae extract/Staphylococcal enterotoxin B (Der f/SEB) induction experiments were performed on (a–e) FcεRIα−/- and (f) TNF-α−/- mice. (a, f) Clinical skin scores. (b–d) Thicknesses of epidermis, dermis, and total skin layers, and (e) inflammatory cell infiltration for these mice are also shown. (b) Hematoxylin and eosin (H&E) staining and (d) immunofluorescence microscopy were performed on naive and lesional skin tissues in FcεRIα−/- mice. *P<0.05. AD, atopic dermatitis; Col, collagen; Ecad, E-cadherin; HPF, high power field; K, keratin; Lor, loricrin; WT, wild type. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 The T helper type 2 (Th2)–promoting cytokine thymic stromal lymphopoietin (TSLP) contributes to skin inflammation. (a) Expression of TSLP (red) before (upper) and after (lower) dermatitis induction with Dermatophagoides farinae extract/Staphylococcal enterotoxin B (Der f/SEB) in wild-type (WT) mice was revealed by immunofluorescence microscopy. Also shown are enlarged images of the areas indicated by rectangles as well as negative control without primary antibody. Bar=100μm. (b–f) Der f/SEB induction experiments were performed on TSLPR−/- mice. (b) Clinical skin scores, (c, d) thicknesses of epidermis, dermis, and total skin layers, and (e) inflammatory cell infiltration are shown. (c) Hematoxylin and eosin (H&E) staining and (f) immunofluorescence microscopy were performed on naive and lesional skin tissues in TSLPR−/- mice. **P<0.01 and ***P<0.001 by Student’s t-test. AD, atopic dermatitis; Col, collagen; Ecad, E-cadherin; HPF, high power field; K, keratin; Lor, loricrin. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

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