1. Long-Distance Migration and Colonization of Transplanted Neural Stem Cells 
Oswald Steward, Kelli G. Sharp, Kelly Matsudaira Yee 
Cell 
Volume 156, Issue 3, Pages (January 2014)
DOI: /j.cell
Copyright © 2014 Elsevier Inc. Terms and Conditions

2. Figure 1
Migration and Colonization of NSCs from Grafts Placed at the Site of a Complete Spinal Cord Transection at T3
(A) Horizontal section through the lesion site immunostained for green immunofluorescence (GFP). The main body of the graft at T3 is evident on the right side of the image. Ectopic cell masses are evident in the central canal rostral to the injury.
(B) Image of GFP-labeled axons streaming from the graft in (A). Section was stained using DAB as the chromogen. Arrow indicates GFP-positive cells.
(C) Section through the cervical spinal cord at about C8 immunostained for GFP reveals a mass of graft-derived cells in the central canal (section stained using DAB as the chromogen).
(D) Ectopic colony adhered to the dorsal part of the spinal cord at T12 overlying the dorsal horn and dorsal root entry zone (DREZ) (section stained using DAB as the chromogen).
(E) High-magnification view of the rostral-most mass in the central canal in (A) stained using DAB as the chromogen. Note axons (ax) in a halo around the mass.
(F) Ectopic colony in the floor of the fourth ventricle adhered to the dorsal brainstem tegmentum.
(G) The same mass shown in (F) immunostained using DAB as the chromogen. Note axons (ax) extending toward the nucleus of the solitary tract (NST).
(H) higher-magnification view of axons in a section near the one shown in (G) extending along the dorsal part of the tegmentum.
(I) Same section illustrated in (F) immunostained for GFAP.
(J) Cross-section through the cervical region coimmunostained for GFP to reveal graft-derived cells and MAP2 to reveal dendrites. Brightness of the green channel is attenuated to allow visualization of MAP2 immunofluorescence.
(K) Ectopic mass in the fourth ventricle immunostained for Ki67.
Cell  , DOI: ( /j.cell )
Copyright © 2014 Elsevier Inc. Terms and Conditions