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Anti-Ferredoxin reductase

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Presentation on theme: "Anti-Ferredoxin reductase"— Presentation transcript:

1 Anti-Ferredoxin reductase
213 Science Bldg. Ewha University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul, Korea Tel: / Fax: POLYCLONAL ANTIBODY Anti-Ferredoxin reductase Catalog number: LF-PA0003 Background: Ferredoxin reductase is a ubiquitous flavoenzyme, containing noncovalently bound FAD as a prosthetic group (1). It plays a role in delivering NADPH or low potential one-electron donors such as ferredoxin and flavodoxin to redox-based metabolisms in plastids, mitochondria and bacteria (2). In mammals, ferredoxin reductase is loosely associated with the inner mitochondrial membrane and receives electrons from NADPH. These electrons are transferred to ferredoxin which shuttles electrons to cytochrome P450 in the adrenal cortex mitochondrial steroid hydroxylation systems (3). 47.5 25 32.5 62 Mr(kDa) 1 2 3 4 5 IMMUNOBLOT ANALYSIS of cell lysates; Lane 1: Mouse Brain Lane 2: Mouse Liver Lane 3: HeLa cell lysates Lane 4: 293T cell lysates Lane 6: Jurkat cell lysates Immunogen: Recombinant human fragment protein purified from E.coli Applications: Western blotting (1:2000) Immunoprecipitation was not tested Host: Rabbit Product: 200ug IgG in 200ul volume of PBS containing 50% glycerol. Background Reference: 1) Carrillo, N., and Ceccarelli, E. D. (2003) Eur. J. Biochem. 270, 2) Lambeth, J. D. and Kamin, H. (1977) J. Biol. Chem. 252, 3) Lin, D. et al. (1990) Proc. Natl. Acad. Sci. USA. 87, Positive control: HeLa cell lysates Concentration: 1 mg/ml Storage: stable for 2 years at -20℃ from date of shipment. Human Mouse Rat Species cross reactivity FOR RESEARCH PURPOSE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.


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