1. Replacement of sodium with choline in slow-cooling media improves human ovarian tissue cryopreservation 
Riccardo Talevi, Vincenza Barbato, Valentina Mollo, Ilaria Fiorentino, Cristoforo De Stefano, Fabio Maria Guarino, Roberto Gualtieri 
Reproductive BioMedicine Online 
Volume 27, Issue 4, Pages (October 2013)
DOI: /j.rbmo
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2. Figure 1
Human primordial/primary follicle grading of grade-1 (A, D, G, J), grade-2 (B, E, H, K) and grade-3 (C, F, I, L) human ovarian follicles. (A–C) Histology, haematoxylin/eosin staining. (D–L) Immunohistochemical localization of apoptotic markers: p53 (D–F), p21 (G–I) and Apaf-1 (J–L) immunohistochemical labelling. Bar=10μm.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
Distribution of grade-1–3 follicles in fresh and cryopreserved ovarian tissue. C=fresh control; P-Na=PrOH-Na-based slow cooling; P-Ch=PrOH-choline-based slow cooling; D-Na=DMSO-Na-based slow cooling; D-Ch=DMSO-choline-based slow cooling. ∗P<0.05 versus fresh control; ∗∗P<0.01 versus fresh control.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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4. Figure 3
Transmission electron micrographs of fresh (A, B) and cryopreserved (C–J) ovarian tissue slow cooled with PrOH-Na (C, D), PrOH-choline (E, F), DMSO-Na (G, H) and DMSO-choline (I, J). Asterisks indicate swollen mitochondria; arrowheads indicate degenerated mitochondria. Bars=10μm (A, C, E, G), 2μm (B, D, F, H, J), 3μm (I).
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5. Figure 4
Proportion of damaged mitochondria in oocytes in fresh and cryopreserved ovarian tissue. C=fresh control; P-Na=PrOH-Na-based slow cooling; P-Ch=PrOH-choline-based slow cooling; D-Na=DMSO-Na-based slow cooling; D-Ch=DMSO-choline-based slow cooling. ∗P<0.05 versus fresh control; ∗∗P<0.01 versus fresh control; ##P<0.01 versus same protocol with sodium-based media.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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6. Figure 5
Transmission electron micrographs of fresh (A, D) and cryopreserved (B, C, E, F) ovarian stroma in samples slow cooled with PrOH-Na (b), PrOH-choline (e), DMSO-Na (c) and DMSO-choline (f). Asterisks indicate intermediate stages of stromal cell necrosis. Bars=10μm (A), 2μm (B–F).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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